Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were evaluated using a chemistry analyzer (Hitachi, Osaka, Japan). Hepatic malondialdehyde (MDA) levels were analyzed using a MDA measurement assay kit (Sigma-Aldrich). Hepatic amounts of GSH and oxidized GSH (GSSG) were evaluated using a glutathione detection assay kit (Abcam). Hepatic concentrations of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were measured using ELISA kits (R&D Systems, Minneapolis, MN). All analyses were carried out following the manufacturers' protocols.
Glutathione detection assay kit
Manufactured by Abcam
Sourced in United States
The Glutathione Detection Assay Kit is a quantitative colorimetric assay designed to measure total glutathione (GSH) levels in various sample types, including cell lysates, tissue homogenates, and other biological samples. The kit utilizes a proprietary reaction that produces a colored product, the intensity of which is proportional to the total glutathione concentration in the sample.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using glutathione detection assay kit
1
Biochemical Markers of Liver Injury
2
Intracellular GSH Assessment in Treated Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The changes of total intracellular GSH in treated with respect to untreated cells was assessed by using Glutathione Detection Assay Kit (Fluorometric, ab65322, Abcam, MA, USA). Cells were incubated with phendione or cuproindione at IC50 concentration for 24 hrs, and lysates obtained by addition of the lysis buffer provided in the kit. After an incubation on ice for 30 min, the lysates were centrifuged (10 min at 14,000 r.p.m.), the supernatants collected and processed according to the kit protocol, to eventually measure fluorescence (excitation/emission wavelengths = 380/461 nm).
3
Oxidative Stress Markers in Diabetes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Diabetes is associated with increased oxidative stress [21 (link), 22 (link)]. SOD [23 (link), 24 (link)], AST [25 (link), 26 (link)], ALT [27 (link), 28 (link)], and GSH are associated with oxidative stress [29 (link)–34 (link)]. Here, the levels of SOD, AST, ALT, and GSH were measured. AST and ALT were measured by an automated clinical chemistry analyzer (Indianapolis, IN, USA). The activity of SOD was measured by Superoxide Dismutase Activity Assay Kit (Cat. number ab65354 from Abcam). GSH was measured by Glutathione Detection Assay Kit (Cat. number ab65322 from Abcam).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!