Agar powder for plate culture and cobalt chloride

The high-performance liquid chromatography (HPLC) system used was composed of a Waters 717 plus Autosampler, a Waters 600 Controller, a Waters 996 Photodiode Array Detector and a Waters Millog workstation (Waters, Shinagawaku, Tokyo, Japan). Optical rotations were recorded on a 341 polarimeter (Perkin-Elmer, Shanghai, China). ¹H-NMR (600 MHz) and ¹³C-NMR (150 MHz) spectra were measured at 25 °C on an Agilent 600MHz DD2(DirectDrive2) spectrometer (Agilent, Beijing, China) with TMS as internal standard. ESIMS were recorded on a 6460 Triple Quad LC/MS (Agilent, Beijing, China). Prep. HPLC was performed on an Agilent-1100 system equipped with a Venusil MP-C18 column (10 mm) 250 mm (Agela Technologies, Shanghai, China). Sephadex LH-20 (Amersham, Piscataway, NJ, USA) was used for column chromatography. The organic solvents used in chromatographic separation were of analytical grade purchased from Sayfo Technology (Tianjin China) and chromatographic grade for HPLC analysis purchased from Tedia, USA. Deionized water was prepared by Reverse osmosis Milli-Q water (18 MΩ) (Millipore, Bedford, MA, USA) and used for all solutions and dilutions. Agar powder for plate culture and cobalt chloride was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

The high-performance liquid chromatography (HPLC) system used was composed of a Waters 717 plus Autosampler, a Waters 600 Controller, a Waters 996 Photodiode Array Detector, and a Waters Millog workstation (Waters, Shinagawa, Tokyo, Japan). Optical rotations were measured in methanol on a PerkinElmer-341 polarimeter. The IR spectra were run on a NicoletAvatar-360FT-IR spectrometer. ¹H NMR (500 MHz) and ¹³C NMR (125 MHz) spectra were measured at 25°C on a Bruker AVANCE DMX 500 NMR spectrometer with TMS as internal standard. CD spectra were measured on a JASCO J-715 (JASCO) spectropolarimeter. UV spectra were also recorded in methanol on a Shimadzu UV2550. ESIMS were recorded on an Agilent 6460 Triple Quad LCMS. Preparative HPLC was performed on a ChuangXinTongHeng system equipped with a Venusil MP-C18 column (10 mm × 250 mm, Agela Technologies, Tianjin, China). The organic solvents used in chromatographic separation were of analytical grade purchased from Sayfo Technology (Tianjin China) and chromatographic grade for HPLC analysis purchased from Tedia, United States. Deionized water was prepared by reverse osmosis Milli-Q water (18 MW; Millipore, Bedford, MA, United States) and used for all solutions and dilutions. Agar powder for plate culture and cobalt chloride was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).