Latex beads with a mean particle size of 3 μm (Sigma) were washed with sterile DPBS (Gibco), resuspended in DPBS, and incubated with 1 μg per 107 beads of CD21/CD35 monoclonal antibody (clone 4E3; eBioscience), IgM rat anti-mouse antibody (clone II/41; BD Biosciences), or a combination of both for 2 h at room temperature under continuous mixing on a rotating wheel. As controls, beads were either uncoupled or coupled to the isotype control antibody rat IgG2a kappa isotype control (clone eBR2a; eBioscience). After incubation, the antibody-coupled beads were washed and resuspended in complete IMDM (Gibco, Invitrogen) before incubation with the B cells at an MOI of 7 for 5 h.
Rat igg2a kappa isotype control clone ebr2a
Manufactured by Thermo Fisher Scientific
The Rat IgG2a kappa isotype control (clone eBR2a) is a laboratory reagent used in flow cytometry and other immunoassays. It is a purified rat immunoglobulin of the IgG2a subclass and kappa light chain isotype, and serves as a negative control for experiments involving rat IgG2a antibodies.
Automatically generated - may contain errors
Lab products found in correlation
Latex bead, by Merck Group (2 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions)
Cd21 cd35 monoclonal antibody clone 4e3, by Thermo Fisher Scientific (2 mentions)
Igm rat anti mouse antibody clone 2 41, by BD (2 mentions)
Complete imdm, by Thermo Fisher Scientific (2 mentions)
2 protocols using rat igg2a kappa isotype control clone ebr2a
1
Latex Bead Conjugation for B cell Activation
2
Latex Bead Conjugation for B cell Activation
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Latex beads with a mean particle size of 3 μm (Sigma) were washed with sterile DPBS (Gibco), resuspended in DPBS, and incubated with 1 μg per 107 beads of CD21/CD35 monoclonal antibody (clone 4E3; eBioscience), IgM rat anti-mouse antibody (clone II/41; BD Biosciences), or a combination of both for 2 h at room temperature under continuous mixing on a rotating wheel. As controls, beads were either uncoupled or coupled to the isotype control antibody rat IgG2a kappa isotype control (clone eBR2a; eBioscience). After incubation, the antibody-coupled beads were washed and resuspended in complete IMDM (Gibco, Invitrogen) before incubation with the B cells at an MOI of 7 for 5 h.
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