Uric acid assay kit

Manufactured by Nanjing Jiancheng

Sourced in China

The uric acid assay kit is a laboratory tool used to measure the concentration of uric acid in a sample. It provides a quantitative assessment of uric acid levels through a colorimetric reaction. The kit includes the necessary reagents and instructions to perform the analysis.

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Lab products found in correlation

Creatinine assay kit, by Nanjing Jiancheng (1 mentions) Phosphate assay kit, by Nanjing Jiancheng (1 mentions) Magnesium assay kit, by Nanjing Jiancheng (1 mentions) Sodium azide, by Sinopharm (1 mentions) Calcium assay kit, by Nanjing Jiancheng (1 mentions) Total protein assay kit, by Nanjing Jiancheng (1 mentions) Triglyceride assay kit, by Nanjing Jiancheng (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Isopropanol, by Thermo Fisher Scientific (1 mentions) Creatinine kit, by Nanjing Jiancheng (1 mentions) Bio plex magpix system, by Bio-Rad (1 mentions) Bio plex pro mouse cytokine grp 1 panel 23 plex kit, by Bio-Rad (1 mentions) Uric acid sodium salt, by Merck Group (1 mentions) Potassium oxonate, by Yuanye Bio-Technology (1 mentions)

Close spelling variants of this product

Uric acid kits (4 citations) Uric acid (3 citations)

7 protocols using uric acid assay kit

Urine Analysis in Rat Metabolic Cages

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On Day 0 (for identifying the model establishment) or Day 28 (for evaluating the effects of Huashi Pill), the rats were fed in metabolic-cages and the 24-hour urine was harvested by incubating with 0.02% sodium azide (SinoPharm Co., Ltd., Shanghai, China) to prevent the appearance of bacteria. The urinary pH and urinary volume were evaluated and then the urine was used for the other urine biochemical analysis. The urine protein (urine protein quantitative kit, Cat. No. C035-2, Nanjing Jiancheng Bioengineering Inst., Nanjing, China), uric acid (uric acid assay kit, Cat. No. C012-1, Nanjing Jiancheng Bioengineering Inst.), calcium (calcium assay kit, Cat. No. C004-3, Nanjing Jiancheng Bioengineering Inst.), magnesium (magnesium assay kit, Cat. No. C005, Nanjing Jiancheng Bioengineering Inst.), phosphorus (phosphate assay kit, Cat. No. C006, Nanjing Jiancheng Bioengineering Inst.) were examined by utilizing the commercial kit on the semiautomatic photometer (Mode: 750, Hitachi, Tokyo, Japan) following to the instructions of the manufacturers.
Moreover, the levels of serum urea nitrogen (BUN) and creatinine (Cr) were also evaluated using the urea assay kit (Cat. No. C013-1, Nanjing Jiancheng Bioengineering Inst.) and creatinine assay kit (Cat. No. C011-2, Nanjing Jiancheng Bioengineering Inst.), according to the manufacturer’s instructions.

Yang A., Guo H., Fu M, & Liu M. (2019). Inhibitive Effects of Huashi Pill on Formation of Renal Stones by Modulating Urine Biochemical Indexes and Osteopontin in Renal Stone Rat Models. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 25, 8335-8344.

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Biomarker Quantification Protocol

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PO (R27A7X13777) was purchased from Shanghai Yuanye Biotechnology Co., Ltd. Uric acid assay kit (20170316), total protein assay kit (20170321), triglyceride assay kit (20170316), and creatinine kit were purchased from Nanjing Jiancheng Bioengineering Institute (China). HPLC-grad solvents (Formic acid, Isopropanol, acetonitrile) were purchased from Fisher (USA). Chloroform and methanol are all analytical regents.

Yang F., Liu M., Qin N., Li S., Yu M., Wang C, & Ma Q. (2019). Lipidomics coupled with pathway analysis characterizes serum metabolic changes in response to potassium oxonate induced hyperuricemic rats. Lipids in Health and Disease, 18, 112.

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Measurement of Circadian Cytokine Profiles

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Mouse blood samples were centrifuged at 4,500 × g for 10 min to obtain the serum, which was diluted 1:4 with the dilution solution. The diluted solution (50 μL) was tested using a Bio-Plex Pro Mouse Cytokine Grp I Panel 23-plex kit (Bio-Rad, Austin, TX, USA) at Wayne Biotechnology (Shanghai) Co., Ltd. according to a previous report (19 (link)) with a bead suspension platform (Bio-Plex MAGPIX System, Bio-Rad). Considering the circadian rhythm of melatonin secretion, we collected serum samples at 4 am (GMT+8). Mouse melatonin levels were measured using enzyme-linked immunosorbent assay kits (ELISA; Yuanxin Biotechnology Co., Ltd., Shanghai, China), and T-tau, interleukin (IL)-6, MCP-1, tumor necrosis factor (TNF)-α, and LCN2 levels were also measured using ELISA kits (Longdeng Biotechnology Co., Ltd., Shanghai, China). The uric acid serum level was tested using the uric acid assay kit (Nanjing Jiancheng Biological Engineering Research Institute, China).

Zhang B., Chen T., Cao M., Yuan C., Reiter R.J., Zhao Z., Zhao Y., Chen L., Fan W., Wang X., Zhou X, & Li C. (2022). Gut Microbiota Dysbiosis Induced by Decreasing Endogenous Melatonin Mediates the Pathogenesis of Alzheimer’s Disease and Obesity. Frontiers in Immunology, 13, 900132.

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Rumen Fluid Analysis of Lambs

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At the end of the trial, five lambs from each group were randomly selected and euthanized via electrical stunning. Within 30 min postmortem, rumen fluid was collected and filtered through a four-layer cheesecloth, and the pH value was determined by using a portable pH meter immediately (PHS-3C, Shanghai, China). At the same time, 1 ml of ruminal fluid was preserved at −80° for DNA extraction; other samples were processed to analyze VFA, microbial protein (MCP), and ammonia–N (NH₃-N). The concentrations of VFA were determined by a gas chromatograph (Agilent Technologies 7820A, USA) based on the method reported previously (14 (link)); the ruminal MCP concentration was detected using a spectrophotometric method, and the concentration of NH₃-N was detected using a uric acid assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's instructions.

Chen H., Guo B., Yang M., Luo J., Hu Y., Qu M, & Song X. (2021). Response of Growth Performance, Blood Biochemistry Indices, and Rumen Bacterial Diversity in Lambs to Diets Containing Supplemental Probiotics and Chinese Medicine Polysaccharides. Frontiers in Veterinary Science, 8, 681389.

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Quantification of Uric Acid in Rat Feces and Urine

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An appropriate amount of frozen rat feces was collected, added proportionally to the PBS solution, and then dissolved in a 60 °C water bath. The sample was vortexed for 1 min and centrifuged at 1000 rpm for 5 min. The supernatant was then collected for further analysis. Frozen rat urine samples were thawed in a 60 °C water bath and diluted with distilled water until they were free of urate crystals. All samples were assayed according to the instructions of the uric acid assay kit supplied by Jiancheng (Nanjing, China).

Zhu W., Bi S., Fang Z., Iddrisu L., Deng Q., Sun L, & Gooneratne R. (2024). Priestia megaterium ASC-1 Isolated from Pickled Cabbage Ameliorates Hyperuricemia by Degrading Uric Acid in Rats. Microorganisms, 12(4), 832.

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Rumen Fluid Analysis of Beef Cattle

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At the end of the trial, one cattle from each replicate, a total of 12 beef cattles were randomly selected for slaughtering and rumen fluid was then collected through a four-layer cheesecloth. Slaughter was carried out according to the cattle slaughter operation procedures of China, GB/T19477-2004. The pH value was determined by a portable pH meter immediately (PHS-3C, Shanghai, China). At the same time, storing 1 ml of rumen fluid was preserved at –80°C in sterile cryovials and stored in liquid nitrogen for further DNA extraction; other samples were processed to analyze VFA, microbial protein (MCP), and ammonia–N (NH₃-N). The concentrations of VFA were determined by a gas chromatograph (Agilent Technologies 7820A, USA) based on the method reported previously (Zhao et al., 2017 (link)); the ruminal MCP concentration was detected using a spectrophotometric method, and the concentration of NH₃-N was detected using a uric acid assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions.

Zou B., Long F., Xue F., Qu M., Chen C., Zhang X, & Xu L. (2022). Alleviation effects of niacin supplementation on beef cattle subjected to heat stress: A metagenomic insight. Frontiers in Microbiology, 13, 975346.

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Uric Acid Regulation in Animal Models

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Potassium oxonate was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Uric acid sodium salt was obtained from Sigma-Aldrich (St. Louis, MO, USA). The uric acid assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Enzyme-linked immunosorbent assay (ELISA) kits for TNF-α, IL-1β, and IL-6 were obtained from Cloud-Clone Corp. (Wuhan, China). All other reagents used were standard laboratory reagents of analytical grade and were purchased locally.

Yao R., Geng Z., Mao X., Bao Y., Guo S., Bao L., Sun J., Gao Y., Xu Y., Guo B., Meng F, & Cui X. (2020). Tu-Teng-Cao Extract Alleviates Monosodium Urate-Induced Acute Gouty Arthritis in Rats by Inhibiting Uric Acid and Inflammation. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 3095624.

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