EVs were labeled with membrane marking dye PKH67 green fluorescence (Sigma, St Louis, MO, USA). EVs secreted by SNU-1 cells were labeled with PKH67 dye. The labeled EVs were co-cultured with NCI-N87 cells for 30 min, 2 h, and 24 h, respectively. Then NCI-N87 cells were immobilized with 4% paraformaldehyde. Subsequent to 10-min nuclei staining with 4′,6-diamidino-2-phenylindole (DAPI, C1025, 10 μg/mL, Beyotime, Nantong, China), uptake of labeled EVs by recipient GC cells was observed by Nikon eclipse fluorescence microscope (Nikon, Tokyo, Japan).
Pkh67 green fluorescence
Manufactured by Merck Group
Sourced in United States
PKH67 green fluorescence is a lipophilic membrane labeling dye that can be used to stain cells or cellular components. The dye incorporates into the lipid regions of the cell membrane, allowing for the visualization and tracking of labeled cells or structures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pkh67 green fluorescence
1
Labeling and Tracking Extracellular Vesicles
2
Neutrophil NET Capture of E. coli
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Neutrophils (1 × 106 cells·mL−1) were cultured for 1 h in a serum‐free condition to induce the formation of NETs. Escherichia coli cells (ATCC25922; ATCC, Manassas, VA, USA) were labeled with PKH67 green fluorescence (Sigma‐Aldrich), added to NETs induced by the serum‐free culture condition at a multiplicity of infection (MOI) of 50, and cultured for 15 min. Cells were then washed five times with PBS and fixed with 5% formaldehyde. DNA was stained with DAPI and cells were visualized by fluorescence microscopy, as described above.
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