Silica gel type g

Isolation commenced after antimalarial activity of the leaf latex of A. megalacantha was established. Isolates were obtained from leaves latex using preparative thin layer chromatography (TLC) prepared by coating the glass plate with silica gel type G (Size: 10-40 μ, Sigma-Aldrich, USA) at 0.5 mm thickness in the laboratory of Pharmacognosy department with the automatic TLC coater (CAMAG, Germany). The dried leaves latex was dissolved in methanol and applied directly as a band to the coated preparative TLC (20 cm × 20 cm) over one side of the plate. Chloroform and methanol mixture (4:1) were used as a solvent system to develop the chromatogram [22 , 24 , 28 (link)]. The isolates were checked for their purity using silica gel over TLC plate (0.25 mm thickness).

The chemicals, reagents, and drugs listed below were used to perform the experiments: chloroform with batch number V3M521074A (CALRO EBRA reagents, France;), methanol with batch number V4H4376174I (CALRO EBRA reagents, France), silica gel type G with batch number 126K0028 (Sigma-Aldrich, USA), Giemsa stain, immersion oil, tween 80, chloroquine phosphate (Addis Pharmaceutical Factory PLC, Adigrat, Ethiopia), sodium citrate, distilled water, and normal saline (0.9%). All the chemicals and reagents used were analytical grade and purchased from market.
Drying oven (GENLAB WIDNES, England), TLC glass plate, TLC jar (CAMAG, Germany), Automatic TLC coater (CAMAG, Germany), UV cabinet (CAMAG, Germany), Compound Microscope (Olympus, Germany), oral gavages, stop watch, electronic weighing balance (Adventurer OHAUS, China), and digital thermometer were also used to conduct the study.