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Pierce 660 nm ready to use protein assay reagent

Manufactured by Thermo Fisher Scientific
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The Pierce-660 nm ready-to-use Protein Assay Reagent is a colorimetric solution designed for the quantification of protein concentrations. This ready-to-use reagent provides a simple and accurate method for determining protein levels in solution.

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Lab products found in correlation

Sandwich elisa, by Fortis Life Sciences (2 mentions) Ascent v 2, by Thermo Fisher Scientific (2 mentions)

2 protocols using pierce 660 nm ready to use protein assay reagent

1

Quantifying Immunoglobulins in Mucosal Samples

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The concentration of IgA and/or IgM in SMG, GW, CC, and feces were determined by a sandwich ELISA (Bethyl Laboratories Inc., Montgomery, TX, United States), as previously explained (36 (link)). Absorbance was quantified on a microplate photometer (Labsystem Multiskan, Helsinki, Finland) and data were interpolated by Ascent v.2.6 software (Thermo Fisher Scientific) according to the respective standard curves. The Ig content in SMG, CC and feces was normalized by the total protein concentration, which was determined using the Pierce-660 nm ready-to-use Protein Assay Reagent (Thermo Fisher Scientific) following the manufacturer’s instructions.
Ruiz-Iglesias P., Massot-Cladera M., Rodríguez-Lagunas M.J., Franch À., Camps-Bossacoma M., Castell M, & Pérez-Cano F.J. (2022). A Cocoa Diet Can Partially Attenuate the Alterations in Microbiota and Mucosal Immunity Induced by a Single Session of Intensive Exercise in Rats. Frontiers in Nutrition, 9, 861533.
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2

Quantification of Immunoglobulin Levels

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The concentrations of IgG, IgM and IgA in MLNL culture supernatants, GW, SMG or CC homogenates were quantified by a sandwich ELISA (Bethyl Laboratories Inc., Montgomery, TX, USA). After assay development as previously described76 (link), absorbance was measured on a microplate photometer (Labsystems Multiskan, Helsinki, Finland) and data were analysed by Ascent v.2.6 software (Thermo Fisher Scientific, S.L.U, Barcelona, Spain) according to the respective standard curves. The immunoglobulin content in SMG and CC was normalized by total protein concentration which was measured using the Pierce-660 nm ready-to-use Protein Assay Reagent (Thermo Fisher Scientific). Changes in each Ig concentration are expressed considering the SED group mean value as 1.
Ruiz-Iglesias P., Estruel-Amades S., Camps-Bossacoma M., Massot-Cladera M., Castell M, & Pérez-Cano F.J. (2020). Alterations in the mucosal immune system by a chronic exhausting exercise in Wistar rats. Scientific Reports, 10, 17950.
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