Sea079ra

Manufactured by Cloud-Clone

Sourced in United States

SEA079Ra is a laboratory equipment product. It is designed for specific scientific and research applications. The core function of this product is to perform a particular type of analysis or measurement, but a detailed description cannot be provided in an unbiased and factual manner without potential extrapolation.

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Lab products found in correlation

Sea133ra, by Cloud-Clone (3 mentions) Sea563ra, by Cloud-Clone (2 mentions) Sea056ra, by Cloud-Clone (1 mentions) Sea084ra, by Cloud-Clone (1 mentions) A001 1 2, by Nanjing Jiancheng (1 mentions) A006 1 1, by Nanjing Jiancheng (1 mentions) A003 1 2, by Nanjing Jiancheng (1 mentions) A044 1 1, by Nanjing Jiancheng (1 mentions) Glutathione (gsh), by Nanjing Jiancheng (1 mentions)

5 protocols using sea079ra

Cytokine Levels in Serum and Culture

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The levels of IL-1β (Cloud-Clone Corp, SEA563Ra, Wuhan, China), TNF-α (Cloud-Clone Corp, SEA133Ra), IL-10 (Cloud-Clone Corp, SEA056Ra), IL-6 (Cloud-Clone Corp, SEA079Ra) in serum and cell culture supernatant were detected according to the instructions.

Fu Z., Zhang Z., Wu X, & Zhang J. (2020). Hydrogen-Rich Saline Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Endothelial Dysfunction by Regulating Autophagy through mTOR/TFEB Signaling Pathway. BioMed Research International, 2020, 9121894.

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Evaluation of NLRP3, Cytokines in Rat Pancreas

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Pancreatic homogenate samples were used for the evaluation of rat nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing protein 3 (NLRP3) (Cat. No. KCD04232, Beijing AVIVA Systems Biology co., China), TNF-α (Cat. No abx050220, Abbexa, Ca, UK), IL-1β and IL-6 (Cat. No SEA563Ra and SEA079Ra, respectively, Cloud-clone Corp., TX, USA) by commercially available ELISA kits, following the instructions of the manufacturer.

Awadeen R.H., Boughdady M.F., Zaghloul R.A., Elsaed W.M., Abu Hashim I.I, & Meshali M.M. (2023). Formulation of lipid polymer hybrid nanoparticles of the phytochemical Fisetin and its in vivo assessment against severe acute pancreatitis. Scientific Reports, 13, 19110.

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Cytokine and Adipokine Quantification

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The serum levels of IL-6, TNF-α, leptin, and adiponectin were determined by the sandwich enzyme immunoassay technique. The commercial rat ELISA kits for IL-6 (SEA079Ra), TNF-α (SCA133Ra), leptin (SEA084Ra), and adiponectin (SEA605Ra) were purchased from Cloud-Clone Corporation Inc. (Katy, TX, 77494, USA). The technique of the assay was according to the manufacturer's instructions.

AlNafea H.M, & Korish A.A. (2021). Activation of the Peroxisome Proliferator-Activated Receptors (PPAR-α/γ) and the Fatty Acid Metabolizing Enzyme Protein CPT1A by Camel Milk Treatment Counteracts the High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease. PPAR Research, 2021, 5558731.

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Cytokine and Oxidative Stress Evaluation

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Cytokines was correlated tightly with the occurrence of the infection, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1), while myeloperoxidase (MPO), malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) were correlated with oxidative stress reaction, which was often occurred in UC. So here we measured this cytokinesis to evaluate the efficacy of the treatment. IL-6 (SEA079Ra; Cloud-Clone Corp) and TNF-α (SEA133Ra; Cloud-Clone Corp) were quantified using commercially available ELISA kits. Standard curves are shown in Supplementary Figure S1D,E. MPO (A044-1-1; Nanjing Jiancheng Bioengineering Institute), GSH (A006-1-1; Nanjing Jiancheng Bioengineering Institute), MDA (A003-1-2; Nanjing Jiancheng Bioengineering Institute) and T-SOD (A001-1-2; Nanjing Jiancheng Bioengineering Institute levels in sera were determined by ELISA assay kits according to the manufacturer’s instructions.

Rong Y., Hong G., Zhu N., Liu Y., Jiang Y, & Liu T. (2021). Photodynamic Therapy of Novel Photosensitizer Ameliorates TNBS-Induced Ulcerative Colitis via Inhibition of AOC1. Frontiers in Pharmacology, 12, 746725.

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Cytokine and Oxidative Stress Measurements

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Rat-specific ELISA kits were applied to determine hepatic and medium tumor necrosis factor α (TNF-α, SEA133Ra, USCN, Cloud-Clone Corp., Wuhan, China), monocyte chemokine protein-1 (MCP-1, SEA087Mi, USCN, Cloud-Clone Corp., Wuhan, China), and hepatic interleukin-6 (IL-6, SEA079Ra, USCN, Cloud-Clone Corp., Wuhan, China) and levels according to the manufacturer's instructions. Absorbance all measured at 450 nm. Hepatic 8-isoprostane level was determined using Cayman: 8-Isoprostane EIA Kit (number 516351, Cayman Chemical, USA) according to the manufacturer's instruction. The absorbance was measured at a wave length of 420 nm.

Zhang Y., Yuan D., Yao W., Zhu Q., Liu Y., Huang F., Feng J., Chen X., Huang Y., Chi X, & Hei Z. (2016). Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation. Oxidative Medicine and Cellular Longevity, 2016, 3919627.

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