Eca 400 mhz nmr spectrometer

Column chromatography was performed using silica gel 60, 230–400 mesh ASTM (Merck, 0.040–0.063mm). Aluminium supported silica gel 60 F₂₅₄ plates 20 x 20cm (absorbent thickness: 0.25 mm) were used for thin layer chromatography (TLC) (Merck, Germany). Preparative thin layer chromatography (PTLC) (Merck, Germany) silica gel 60 F₂₅₄ glass plates 20 x 20cm (absorbent thickness: 0.50 mm) were used for separation of compounds. IR spectrum was recorded using a Perkin-Elmer Spectrum 400 FT-IR Spectrometer with spectroscopic grade chloroform as the solvent. 1D- and 2D-NMR spectra were recorded in chloroform CDCl₃ (Merck, Germany) using JEOL ECA 400 MHz NMR spectrometer. The LCMS-IT-TOF spectra were recorded on a UFLC Shimadzu Liquid Chromatography with a SPD-M20A diode array detector coupled to a IT-TOF mass spectrometer. UV spectra were recorded using a Shimadzu 1650 PC UV-Vis Spectrophotometer with spectroscopic grade methanol (CH₃OH) as solvent. All solvents were of analytical grade and were distilled prior to use.

Analytical TLC was carried out on 60 F₂₅₄ silica gel plates (absorbent thickness: 0.25 mm, Merck, Darrmstadt, Germany). Column chromatography was performed using silica gel (Merck 230–400 mesh, ASTM). IR spectra were recorded using a Perkin-Elmer Spectrum 400 FT-IR Spectrometer (Perkin Elmer, Waltham, MA, USA). NMR spectra were acquired in CD₃OD (Merck) using a JEOL ECA 400 MHz NMR spectrometer (JEOL, Tokyo, Japan). The LCMS-IT-TOF spectra were obtained on a UFLC Shimadzu Liquid Chromatograph with a SPD-M20A diode array detector coupled to an IT-TOF mass spectrometer (Shimadzu, Kyoto, Japan). UV spectra were recorded using a Shimadzu 1650 PC UV-Vis Spectrophotometer (Shimadzu). All solvents were of analytical grade and were distilled prior to use.