HepG2/STING and HepG2/STING C cells were mock-treated or treated with the indicated concentrations of G10 or DSDP for 2 h. The cells were fixed with PBS containing 4% paraformaldehyde followed by incubation with 0.1% Triton X-100 for 20 min. Cells were then blocked and incubated with an antibody against IRF3 (Cell Signaling). Bound primary antibody was visualized by Alexa Fluor 488-conjugated secondary antibody (Invitrogen) (Zhu et al., 2003 (link)). Cell nuclei were stained with DAPI.
Antibody against irf3
Manufactured by Cell Signaling Technology
Sourced in United States
The Antibody against IRF3 is a laboratory reagent produced by Cell Signaling Technology. It is designed to detect the Interferon Regulatory Factor 3 (IRF3) protein, which plays a key role in the activation of type I interferon genes in response to viral infection or other stimuli. This antibody can be used for applications such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of IRF3 in various biological samples.
Automatically generated - may contain errors
2 protocols using antibody against irf3
1
Immunofluorescence Staining of IRF3
2
Antibody Validation for Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibody against IRF-3 was from Cell Signaling Technology (Danvers, MA, USA). Anti-PTEN antibody was from Abmart (Shanghai, China). Anti-green fluorescent protein (GFP) tag antibody was from Proteintech (Rosemont, IL, USA). Antibodies against lamin-B1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were from Bioworld Technology (Visalia, CA, USA). Anti-PCNA antibody was from Huabio (Hangzhou, Zhejiang Province, China). Anti-mouse and rabbit IgG-HRP antibodies were from Biodragon (Beijing, China). Antibody against phospho-IRF-3 (Ser97) was a gift from Dr. Deyin Guo (Sun Yat-sen University, China). The viral analogues polyinosinic-polycytidylic [Poly (I:C)] acid and poly (deoxyadenylic-deoxythymidylic) [Poly (dA:dT)] acid sodium salt were from In vivo Gen (San Diego, CA, USA). The inhibitor of STAT1 signaling Fludarabine (1.5 μM) and potent IKK2 inhibitor SC-514 (100 μM) were from Selleck (Houston, Texas, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!