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Ctm 651415

Manufactured by Horizon Discovery
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The CTM-651415 is a laboratory instrument designed for cell culture applications. It provides precise temperature and humidity control to maintain optimal environmental conditions for cell growth. The core function of this product is to create and maintain a stable and regulated cell culture environment.

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Lab products found in correlation

Ctm 651416, by Horizon Discovery (2 mentions) Sigenome smartpool, by Horizon Discovery (2 mentions) Effectene, by Qiagen (2 mentions)

2 protocols using ctm 651415

1

Targeted Silencing of Key Regulators

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Target-specific pools of four independent small interfering RNA (siRNA) species against human CD98hc (M-003542-02), GEF-H1 (M-009883-01), GEF-H1 3′-UTR (CTM-651415, CTM-651416), eIF4A1 (M-020178-01), eIF4A2 (M-013758-01), CPSF73 (M-006365-00), eEF1ε1 (M-015983-01), and non-targeting siRNA control pool #1 (D-001206-13) (siGENOME SMARTpool) were purchased from Dharmacon (Horizon Discovery). siRNA pools were transfected using Effectene (QIAGEN) at a final concentration of 50 nM for 48 hr unless otherwise stated before subsequent treatments. ARHGEF2/GEF-H1 ORF cDNA construct (OHu26696) and empty vector were purchased from GenScript, and transfected using Effectene (QIAGEN) following manufacturer protocols.
Ho J.J., Cunningham T.A., Manara P., Coughlin C.A., Arumov A., Roberts E.R., Osteen A., Kumar P., Bilbao D., Krieger J.R., Lee S, & Schatz J.H. (2021). Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome. Cell Reports, 37(2), 109806.
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2

Targeted siRNA-mediated knockdown

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Target-specific pools of four independent small interfering RNA (siRNA) species against human CD98hc (M-003542-02), GEF-H1 (M-009883-01), GEF-H1 3′-UTR (CTM-651415, CTM-651416), eIF4A1 (M-020178-01), eIF4A2 (M-013758-01), CPSF73 (M-006365-00), eEF1ε1 (M-015983-01), and non-targeting siRNA control pool #1 (D-001206-13) (siGENOME SMARTpool) were purchased from Dharmacon (Horizon Discovery). siRNA pools were transfected using Effectene (QIAGEN) at a final concentration of 50 nM for 48 hr unless otherwise stated before subsequent treatments. ARHGEF2/GEF-H1 ORF cDNA construct (OHu26696) and empty vector were purchased from GenScript, and transfected using Effectene (QIAGEN) following manufacturer protocols.
Ho J.J., Cunningham T.A., Manara P., Coughlin C.A., Arumov A., Roberts E.R., Osteen A., Kumar P., Bilbao D., Krieger J.R., Lee S, & Schatz J.H. (2021). Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome. Cell Reports, 37(2), 109806.
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