Lyolab 3000

Microwave processing of cow milk was conducted in a Mini WAVE Digestion Module (SCP Science, Montreal, Canada) that operates at a frequency of 2.45 GHz. Cow milk (30 mL) was transferred into a cylindrical quartz reactor vessel (100 mL), and then the milk was processed inside the Mini WAVE, which included 6 chambers designed to accompany the vessel. According to preliminary studies (50–100 °C, 0–10 min), the Mini WAVE was operated at 60, 75, and 90 °C for 1, 3, and 5 min, respectively (Table 1a). The processing time and temperature of the samples were monitored by Infrared Radiation (IR) sensors located at the bottom wall of the treatment chambers. The samples were then freeze-dried (LyoLab 3000, Thermo Scientific, Montreal, Canada) and stored at 4 °C until further analysis. All treatments were carried out in three replicates.

About 5 kg of Passiflora tripartita var. Mollissima fruits were purchased from a local market in Loja (Ecuador). Pulp was manually separated, immediately frozen in encoded plastic bags at −20 °C, and then freeze-dried (Thermo HETO, powerdry LYOLAB 3000; Waltham, MA, USA). Dried samples were ground to a fine powder in a blender mixer (Ika-Werke M20; Staufen, Germany) and stored at −18 °C until extraction.
Before the drying process, soluble solids (° Brix), pH and acidity were determined, and the results are shown in Table 1.

Purified NMed aliquots of 0.5 mL were lyophilised (LyoLab 3000, Heto-Holten, ThermoFisher Scientific, Waltham, MA, USA) in pre-weighed Eppendorf tubes, and the weight yield % was calculated as follows: