Dad was dissolved in MilliQ water and 100 uL of 2 M sodium hydroxide to make a to a 5 mM starting stock. Samples were individually diluted to the desired concentration using 50:50 acetonitrile to phosphate buffered saline (CH3CN/PBS) in triplicate. The absorbance spectrum was measured on a ThermoScientific Genesys 150 UV-Vis spectrometer, using 50:50 CH3CN/PBS as a blank. These absorbances were also measured using a Tecan M1000 plate reader with acridone as a standard.
Genesys 150 uv vis spectrometer
Manufactured by Thermo Fisher Scientific
The Genesys 150 UV-Vis spectrometer is a laboratory instrument used for the analysis of light absorption in the ultraviolet and visible wavelength ranges. It is designed to measure the concentration of chemical substances in a sample by analyzing the absorption of light at specific wavelengths.
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Lab products found in correlation
2 protocols using genesys 150 uv vis spectrometer
1
Quantifying Dad Absorbance Spectra
2
Quantifying Dad Absorbance Spectra
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