Cy 3 conjugated monoclonal goat anti rabbit igg

Immunocytochemistry was performed as described in (22 (link)). Primary antibodies used were rabbit anti-Cleaved Caspase-3 (Asp175) (Cell Signalling, cat no. 9661) at 1:400 dilution and rat anti-Dilp8 at 1:400 dilution (22 (link)). Secondary antibodies used were Cy-3-conjugated monoclonal goat anti-rabbit IgG (Jackson ImmunoResearch, cat. no. 711–165–152) at 1:400 dilution and anti-rat IgG Dylight 488 (Abcam, cat. no. ab96887) at 1:100 dilution. Images were taken with a Leica SP8 confocal microscope. Image stacks and 3D projections were processed in Fiji (http://fiji.sc/Fiji).

Immunocytochemistry was performed essentially as described in (Sullivan et al., 2000 ). Images were taken with a Zeiss Axiovert confocal microscope equipped with a LSM520 Meta. Primary antibodies used were anti-Pacman (Grima et al., 2008 (link)) (1:500), anti-Cleaved Caspase-3 (Asp175) (Cell Signaling, cat no. 9661;1:400), anti-phosphohistone H3 (Ser10) (Cell Signaling, cat. no. 9701; 1:400) and anti-Wingless (4D4) (Developmental Studies Hybridoma Bank; 1:400). Secondary antibodies used were Cy3-conjugated monoclonal goat anti-rabbit IgG (Jackson ImmunoResearch, cat. no. 711-165-152; 1:400), Cy3-conjugated monoclonal Donkey anti-mouse IgG (Jackson ImmunoResearch, cat. no.715-165-151; 1:400) and FITC-conjugated polyclonal goat anti-rabbit (Sigma, cat. no. F9887; 1:200).