Denaturing gel

Manufactured by Bio-Rad

The 4–20% denaturing Gel is a laboratory electrophoresis gel used for the separation and analysis of proteins. It is designed to provide a consistent gradient of denaturing conditions across the gel, allowing for the effective separation of proteins based on their molecular weight.

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Lab products found in correlation

Coomassie brilliant blue r 250, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

SDS–PAGE denaturing gels 15% SDS denaturing gels Bis-Tris XT denaturing 10% SDS polyacrylamide gels Denaturing polyacrylamide gels Tris-HCl denaturing polyacrylamide Ready Gel Non-denaturing gel Mini-Protean TGX denaturing SDS-PAGE gel 10% denaturing acrylamide gel

2 protocols using denaturing gel

Proteomic Analysis of Dbp10 Pre-Ribosome

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10 µL of Dbp10 or Dbp10^R522V pre-ribosomes at an OD260 absorbance of 5.0 were run on a 4–20% denaturing Gel (BioRad) at 160 V for 7 min and stained with Coomassie Brilliant Blue R-250 (Thermo Fisher). The portion of the gel with protein bands was excised and used for subsequent analysis. Semi-quantitative mass-spectrometry data was obtained by the UTSW Proteomics core, analyzed using Proteome Discoverer 2.4 and searched against the yeast protein database from UniProt. Only high-confidence spectra (FDR ≤ 1%) were considered and are listed in Source Data. Selection of RBFs for our plot was based on their presence in known intermediate structures or well-established presences in specific pre-60S intermediates.

Cruz V.E., Weirich C.S., Peddada N, & Erzberger J.P. (2024). The DEAD-box ATPase Dbp10/DDX54 initiates peptidyl transferase center formation during 60S ribosome biogenesis. Nature Communications, 15, 3296.

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Tif6-containing Pre-ribosome Protein Analysis

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3.3 µM of Tif6 containing pre-ribosomes were run on a 4–20% denaturing Gel (BioRad) at 160 V for 7 min and stained with Coomassie Brilliant Blue R-250 (Thermo Fisher). The portion of the gel with protein bands was excised and used for subsequent analysis. Semi-quantitative mass-spectrometry data was obtained by the UTSW Proteomics core, analyzed using Proteome Discoverer 2.4 and searched against the yeast protein database from UniProt. Only high-confidence spectra (FDR ≤ 1%) were considered and are listed in Source Data. Selection of RBFs for our plot was based on their presence in known intermediate structures or well-established presences in specific pre-60S intermediates.

Sekulski K., Cruz V.E., Weirich C.S, & Erzberger J.P. (2023). rRNA methylation by Spb1 regulates the GTPase activity of Nog2 during 60S ribosomal subunit assembly. Nature Communications, 14, 1207.

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