Clx imager system

Parasites were isolated by lysing RBCs in 0.05% saponin in PBS with protease inhibitors (SigmaFast Protease Inhibitor Cocktail). Pelleted parasites were washed with PBS until the supernatant was clear; samples were then boiled in Laemmli buffer for 5 min (for PfMyoJ-smV5^Tet parasites only: incubated at 60 °C for 10 min). The equivalent of 1 mL of culture at 1% schizontemia was run on a 4–20% Tris-glycine-sodium dodecyl sulfate gel and transferred to either a polyvinylidene fluoride or nitrocellulose membrane. Membranes were blocked in Licor Odyssey blocking buffer, incubated with primary antibody, and then incubated in secondary antibodies diluted in blocking buffer. Following TBST-washing, Membranes were scanned on a Licor Odyssey CLx imager system and quantified using volumetric measurement of fluorescence intensity in LiCor Image Studio 4.0. Uncropped blot images are provided in the Source Data file. Dilutions of primary antibodies were: anti-V5 1:1000, anti-MORN1 1:2000, anti-H3 1:2500, anti-LDH 1:1000, anti-Myc 1:1000. Uncropped western blot images are provided in the Source Data file.