Anti phospho dna pkcs ser2056

Manufactured by Abcam

Anti-phospho-DNA-PKcs (Ser2056) is a primary antibody that recognizes the phosphorylated form of DNA-PKcs at serine 2056. DNA-PKcs is a serine/threonine protein kinase that plays a crucial role in the non-homologous end joining (NHEJ) pathway of DNA double-strand break repair.

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DNA-PKcs (17 citations) Anti-DNA-PKcs (10 citations) Anti-PKC (7 citations) Phospho DNA-PKcs (6 citations) Phospho-DNA-PKcs (Ser2056) (4 citations)

2 protocols using anti phospho dna pkcs ser2056

TNKS1BP1 Expression and Knockdown Vectors

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TNKS1BP1 /TAB182 expression vector (PLPC-MYC-TAB182) was kindly provided by Dr. Susan Smith (Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine). PCMV-HA-TNKS1BP1 vector was generated by polymerase chain reaction (PCR) cloning. shRNA-TNKS1BP1-U6/Hygromycin vectors and shRNA-NC-U6/hygromycin vectors were purchased from Genepharma. To obtain the RNA interference resistant TNKS1BP1 expression plasmid (PCMV-HA-shiTNKS1BP1), the region targeted by the TNKS1BP1 shRNA was changed to GGAGAGTTTCTCAAGTCGAGGGAGCGTGGA. Mutagenesis of TNKS1BP1 was done using the Q5 hot start High-fidelity DNA polymerase. DNA-PKcs Inhibitor (Nu7026), PARP inhibitors (3-AB, XAV939), Propidium Iodide (PI) and DAPI were purchased from Sigma. All antibodies were commercial products. Anti-actin, anti-DNA-PKcs, anti-Ku86, anti-PARP-1, anti-TAB182 (TNKS1BP1), anti-Myc (9E10) and anti-HA were purchased from Santa Cruz. Anti-Ku70, anti-pADPr and anti-phospho-DNA-PKcs (Ser2056) were purchased from Abcam. Anti-Phospho-H2AX (Ser139) was purchased from Millipore, AlexaFlour 568-goat anti-rabbit IgG and AlexaFlour 488-goat anti-mouse IgG were purchased from Invitrogen.

Zou L.H., Shang Z.F., Tan W., Liu X.D., Xu Q.Z., Song M., Wang Y., Guan H., Zhang S.M., Yu L., Zhong C.G, & Zhou P.K. (2015). TNKS1BP1 functions in DNA double-strand break repair though facilitating DNA-PKcs autophosphorylation dependent on PARP-1. Oncotarget, 6(9), 7011-7022.

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Monitoring DNA Damage Response Pathways

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The monoclonal antibodies used for this study included: anti-phospho-ATM (Ser 1981) (Cell Signaling Technology, Inc., Danvers, MA; Cat. #4526); anti-phospho-ATR (Cell Signaling Technology, Inc., Danvers, MA; Cat. #2853); anti-phospho-DNA PKcs (Ser2056) (Abcam, Cambridge, MA; Cat. ab18192); anti-phospho-Histone H2AX (Ser139) (Millipore, Mahopac, NY; Cat. #05-636); anti-8-OHdG (Abcam, Cambridge, MA; Cat. ab10802); LC3B (Invitrogen, Inc., Palo Alto, CA; Cat. L10382); NF-κB p65 (Cell Signaling Technology, Inc., Danvers, MA; Cat.4764S); iNOS (Cell Signaling Technology, Inc., Danvers, MA; Cat.13120).

Zhang B., Davidson M.M, & Hei T.K. (2014). Mitochondria regulate DNA damage and genomic instability induced by high LET radiation. Life sciences in space research, 1, 80-88.

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