Unless stated otherwise all materials were obtained from Sigma Aldrich (Poole, Dorset, U.K.) in their highest purity. RPMI, Dulbecco's modified Eagle medium (DMEM), phosphate buffered saline (PBS), foetal calf serum (FCS), Glutamax, penicillin and streptomycin were obtained from Invitrogen (Paisley, U.K.). THP-1 and J774 cells were obtained from the European Collection of Animal Cell Cultures (Salisbury, Wiltshire, U.K.). [γ-32P]-ATP (370MBq/ml, 10 mCi/ml), polyvinylidene difluoride membrane (PVDF) and poly(dI–dC)–poly(dI–dC) was obtained from Amersham Biosciences (Little Chalfont, Buckinghamshire, U.K.). NF-κB Gel Shift Assay System was obtained from Promega (Southampton, Hampshire, U.K.). Goat polyclonal antibodies for p50 (cross reactive with p105), rabbit polyclonal antibodies to p50, p65 or IκBα and actin, donkey anti-goat IgG–horseradish peroxidase (HRP) and supershift antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, U.S.A.). Donkey anti-goat IgG–fluorescein isothiocyanate (FITC) was obtained from AbD Serotec (Oxford, U.K.). Lymphoprep was obtained from Axis-Shield (Oslo, Norway).
Donkey anti goat igg horseradish peroxidase hrp
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Donkey anti-goat IgG–horseradish peroxidase (HRP) is a secondary antibody conjugate used in immunoassays and Western blotting applications. It is composed of donkey-derived antibodies that specifically recognize and bind to goat immunoglobulin G (IgG), coupled with the enzyme horseradish peroxidase (HRP).
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
γ 32p atp, by Cytiva (1 mentions)
Foetal calf serum, by Thermo Fisher Scientific (1 mentions)
Poly di dc poly di dc, by Cytiva (1 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)
Lymphoprep, by Axis-Shield (1 mentions)
Cyclooxygenase 2 cox2, by Cell Signaling Technology (1 mentions)
Iba 1, by Abcam (1 mentions)
2 protocols using donkey anti goat igg horseradish peroxidase hrp
1
NF-κB Activation Assay in THP-1 and J774 Cells
2
Immunohistochemical Staining for Neurodegenerative Markers
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Immunohistochemical staining was performed according to a previously described method (Gu et al. 2015 (link)). The sections were reacted with specific primary antibodies for Aβ (1:300; Abcam, Inc., Cambridge, MA, USA), glial fibrillary acidic protein (GFAP) (1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), ionize calcium-binding adapter molecule 1 (IBA-1) (1:300; Abcam, Inc., Cambridge, MA, USA), inducible nitric oxide synthase (iNOS) (1:300; Novus Biologicals, Inc., Littleton) and cyclooxygenase-2 (COX-2) (1:300; Cell Signaling Technology, Inc., Beverly, MA, USA). Tissues were then incubated with the corresponding conjugated secondary antibodies: goat anti-rabbit, goat anti-mouse, or donkey anti-goat IgG-horseradish peroxidase (HRP) (1:5000; Santa Cruz Biotechnology Inc. Santa Cruz, CA, USA). The sections were evaluated under a light microscope (Microscope Axio Imager.A2, Carl Zeiss, Oberkochen, Germany) (×50 or ×200 or ×400).
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