Neutral buffered formalin fixative solution

The endothelial-specific conditional Krit1 null mice were generated by breeding mice expressing endothelial specific Pdgfb promoter driven tamoxifen-regulated Cre recombinase, CreERT2, in combination with loxP-flanked Krit1 exon 5 (Pdgfb^CreERT2Krit1^fl/fl).^{38 (link)} The endothelial-specific conditional Pdcd10 null mice were generated by breeding mice expression Pdgfb^CreERT2 in combination with LoxP-flanked Pdcd10 exon 4 (Pdgfb^CreERT2Pdcd10^fl/fl). At 1, 2 and 3-day post-birth, mice were intraperitoneally administered 50 μg of tamoxifen (Sigma Aldrich) to induce Cre activity and endothelial Krit1 or Pdcd10 gene inactivation in the littermates bearing the CreERT2. Mice were sacrificed by decapitation for phenotypic analysis on postnatal day 7. Mouse brains were surgically removed and dropped into a 10% neutral buffered formalin fixative solution (Sigma Aldrich). All animal experiments were carried out in compliance with animal procedure protocols approved by the University of California, San Diego IACUC.