Culture supernatants were collected on days 0, 3, 6, 9, 12,15, 18, 21 and 25 and filtered (0.22 μm hydrophilic PTFE, Micro Analytix Pty Ltd, Australia). Filtrates were analysed on a Varian 820-MS inductively coupled plasma mass spectrometer (Melbourne, Australia) for Al, As, B, Cu, Fe, Mo, Ni, Se, Sr, V and Zn at the Advanced Analytical Centre (AAC), James Cook University (JCU). Indium served as the internal standard to correct for matrix effects and instrument drift. For quantification, a series of multi-element standard solutions were used to calibrate the instrument (Taylor et al., 1998 ). An independent multi-element standard was used for quality control; the accuracy achieved was typically within 5%.
820 ms inductively coupled plasma mass spectrometer
Manufactured by Agilent Technologies
Sourced in Australia
The 820-MS inductively coupled plasma mass spectrometer is a laboratory instrument designed for elemental analysis. It utilizes inductively coupled plasma (ICP) technology to atomize and ionize samples, and a mass spectrometer to detect and quantify the constituent elements. The 820-MS provides high sensitivity and accuracy for the determination of trace and ultra-trace elements in a variety of sample types.
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Lab products found in correlation
2 protocols using 820 ms inductively coupled plasma mass spectrometer
1
Trace Element Analysis of Cell Cultures
2
Simultaneous Measurement of Trace Elements
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The concentrations of metals and light metal ions (Al, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mn, Mg, Na, Ni, Pb, Sr, V, and Zn) and metalloids (As, B, Mo, Se) were measured using a Bruker 820-MS Inductively Coupled Plasma Mass Spectrometer (ICP-MS; Al, Ba, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Sr, V, Zn) or a Varian Liberty series II Inductively Coupled Plasma Optical Emissions Spectrometer (ICP-OES; Ca, K, Mg, Na). An external calibration strategy was used for both instruments, where a standard solution of 0.45 µm filtered ADW was used as the vector to calculate the concentration of elements. Collisional Reaction Interface (CRI) was used for As (CRI gas: H2) and V (CRI gas: He), while 82Se isotope was used for Se quantification, to eliminate polyatomic interferences for these elements. A 1% HCl solution was spiked with 1 ppb As, Se and V and measured three times for quality control; recovery between 98.5 and 110% indicated no significant interferences. All analyses were conducted at the Advanced Analytical Centre at JCU, Townsville.
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