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2 protocols using n butanol

1

Investigating Yeast Strain Interactions

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The
two S. cerevisiae strains W15 and ICV-K1
(Sc1 and Sc2, Lalvin, Montreal, Quebec, Canada), T.
delbrueckii
Biodiva (Td, Level, Edwardstown, Australia), M. pulcherrima Flavia (M1, Level, Edwardstown, Australia),
and M. fructicola IOC Gaïa (M2,
Edwardstown, Australia) were kindly provided by Lallemand, Inc. (Montreal,
Quebec, Canada), and S. bayanus (Sb,
Condessa, Viinitialo Melkko, Ltd., Lahti, Finland) was purchased from
a local wine equipment store in Turku, Finland.
Quercetin-3-O-glucoside, cyanidin-3-O-glucoside, and
(+)-catechin were purchased from Extrasynthese (Genay, France). p-Coumaric acid, chlorogenic acid, xylitol, tartaric acid,
quinic acid, fumaric acid, shikimic acid, and galacturonic acid were
purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). Citric acid
and sucrose were purchased from J.T. Baker Chemicals (Leuven, Belgium). n-Butanol was purchased from Riedel-de Haën (Morris
Plains, NJ, U.S.A.), and ethanol was purchased from Altia (Helsinki,
Finland).
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2

Fluorescent Labeling Reagents for Nucleic Acid Visualization

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Sodium dodecylsulfate (SDS, ≥99.0%, Sigma-Aldrich, Germany), n-butanol (>99.5%, Riedel-de-Haën, Germany), α-tocopherol (97%, ABCR, Germany), trisodium citrate (99%, Carl Roth, Germany), gadolinium chloride hexahydrate (>99%, Sigma-Aldrich), irinotecan (98%, ABCR, Germany), sodium adenosine monophosphate (>99%, Sigma-Aldrich, Germany), DUT549P1-aadUTP (Dyomics, Germany), ATTO647N-aadUTP (≥95%, Jena Bioscience, Germany) and fludarabine phosphate (>99%, Sigma-Aldrich, Germany) were used as purchased.
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