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Catalase cat

Manufactured by Solarbio
Sourced in China

Catalase (CAT) is an enzyme that catalyzes the decomposition of hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2). It is a widely distributed enzyme found in nearly all living organisms that are exposed to oxygen, where it functions to protect cells from the toxic effects of hydrogen peroxide.

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2 protocols using catalase cat

1

Cellular Antioxidant Assays of Fatty Acids and Vitamins

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Fetal bovine serum (FBS) was purchased from Gibco (NY14072, USA). RPMI-1640 medium, penicillin/streptomycin liquid (100×), 0.25% trypsin-0.02% EDTA solution and dimethyl sulfoxide (DMSO) were purchased from Solarbio Biotechnology (Beijing, China). VE was obtained from Bide Pharmaceutical Technology Co., Ltd. (Shanghai, China). Lycopene, EPA and DHA were purchased from Acmec Biochemical Co., Ltd. (Shanghai, China). Dichloromethane was purchased from Xilong Science Co., Ltd. (Guangdong, China). Trypan blue stain and Cell Counting Kit (CCK-8) were obtained from Beyotime Biotechnology (Shanghai, China). Commercial assay kits for bicinchoninic acid (BCA) protein quantification, reactive oxygen species (ROS), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were all procured from Solarbio Biotechnology (Beijing, China).
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2

Evaluating Antioxidant Potential of ZSM-5 Zeolites

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Three ZSM-5 zeolites, ZSM-5(27), ZSM-5(85), and ZSM-5(500), with Si:Al molar ratios of 27, 85, and 500, respectively, were purchased from Nankai University Catalyst Co., Ltd. Pectinase preparation (20 U/mg d.w.) was obtained from Sangon Biotech Co., Ltd. (Shanghai). The chemicals 4-nitrophenyl β-D-glucopyranoside (PNPG) and catalase (CAT) and kits for SOD activity detection and glutathione (GSH) content detection were purchased from Beijing Solarbio Science & Technology Co., Ltd. Baicalin (HPLC grade) was purchased from Aladdin Industrial Corporation. RAW264.7 cells were obtained from Peking Union Medical College. Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Beijing Solarbio Science & Technology Co., Ltd. All other chemicals were of analytical grade.
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