Gamma scintigraphy was carried out to confirm the development of sustained release depot at the site of injection following the administration of liquid ZP-LPS formulation. Briefly, ZP was radiolabeled with 99mTechnetium-pertechnetate (Tc-99M) by an in-house developed standard procedure using stannous chloride as reducing agent. Labeling efficiency and stability was confirmed by instant thin layer chromatography.16 (link) ZP-LPS Formulation was then developed using Tc-99M labeled ZP. ZP-LPS formulation (0.3 mL) containing 60 μCi ofTc-99Mwas then injected subcutaneously at the back of the three Sprague-Dawley rats. At pre-designated time points, 15 min, 6 h and 12 h, gamma images were captured by gamma camera (Siemens T2 SPECT-CT) to understand drug release and depot formation.17 (link)
T2 spect ct
Manufactured by Siemens
The T2 SPECT-CT is a medical imaging device that combines single-photon emission computed tomography (SPECT) and computed tomography (CT) technology. It is designed to provide accurate and detailed images of the body's internal structures and functions.
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Lab products found in correlation
2 protocols using t2 spect ct
1
Gamma Scintigraphy for Sustained Release Depot Evaluation
2
Gamma Scintigraphy Confirms Intranasal CONPs Brain Targeting
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The gamma scintigraphy (Figure 3 ) was used to confirm the brain targeting of intranasal CONPs. Considering proof-of-concept study, only three healthy male Sprague Dawley rats of weight approx. 250 ± 30 g, aged 6–7 months were used. Twenty four hours before of the experiment, rats remained in a fasted state. Further, radiolabeled intranasal CONPs (50 µCi of 99mTc) were administered to rats using micro pipette. To understand the brain delivery of CONPs, the images were obtained at 15, 30 and 60 min using gamma camera (Siemens T2 SPECT-CT).
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