Bde 100

BDE-47 [bromine substitution pattern 2,2′,4,4′], BDE-100 [2,2′,4,4′,6], and BDE-153 [2,2′,4,4′,5,5′] were purchased from AccuStandard, Inc. 17β-estradiol (E2) (Sigma-Aldrich Coporation, St. Louis, MO) and dimethyl sulfoxide (DMSO 99.7%; Sigma-Aldrich Corporation [St. Louis, MO]) were purchased.

PBDEs (2,2’,4,4’-tetrabromodiphenyl ether (BDE-47), 2,2’,4,4’,6-pentabromodiphemyl ether (BDE-100), and 2, 2’,4,4’5,5’-hexabromodiphenyl ether (BDE-153)) were purchased from AccuStandard, Inc., New Haven, CT. These were three major PBDE congeners detected in women from the California Teachers Study^{70 (link)}. 17β-Estradiol, progesterone, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich Corporation, St. Louis, MO.

Standard solutions of organochlorine pesticides (OCs) and their metabolites, as well as of organophosphate pesticides (Ops) (see Table 1 for the list of OCs and OPs), were purchased from Restek (Bellefonte, PA, USA). Similarly, standard solutions of polycyclic aromatic hydrocarbons (i.e., chrysene, benzo(α)anthracene, benzo(β)fluoranthene, and benzo(α)pyrene) were purchased from Restek (Bellefonte, PA, USA). Mixed solutions of PCB congeners (see Table 2 for the list) and CB-209, which was used as an internal standard (IS) for PCBs and PAHs, as well as mixed solutions of PBDE congeners (BDE-28; BDE-33; BDE-47; BDE-99; BDE-100; BDE-153; and BDE-154) and fluorobromodiphenyl ether (FBDE), which was used as IS for flame retardants, were purchased from AccuStandard (New Haven, CT, USA). The standard solutions of per- and polyfluoroalkyl substances (PFASs; see Table 3 for the list), as well as the two 13C-labeled IS of MPFNA and MPFOS, were purchased from AccuStandard (New Haven, USA). The QuEChERS ‘SupelTM QuE Citrate (EN) tubes for extraction and SupelTM QuE-ZSEP (EN) tubes for the clean-up step used for analyses of OCs, OPs, PCBs, PAHs, and PBDEs were purchased from Supelco (Sigma-Aldrich, St. Louis, MO, USA). The extraction cartridges used for PFASs analyses (Oasis WAX 3 mL, 60 mg) were provided by Waters (Milford, MA, USA).