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2 protocols using anti actin 60008 1 ig

1

Characterizing Mitochondrial Dynamics Factors

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Anti-GFP (1:1000, 50430-2-AP, ProteinTech), anti-Mff (17090-1-AP; ProteinTech), anti-Drp1 (#8570; CST), anti-Mfn2 (12186-1-AP; ProteinTech), anti-PTPIP51 (20641-1-AP; ProteinTech), anti-calnexin (10427-1-AP; ProteinTech), anti-COXIV (11242-1-AP; ProteinTech), anti-flag (F1804; Sigma), and anti-p97/VCP (10736-1-AP; ProteinTech) were used at 1:1000 dilutions for Western blots. Anti-VAPB (14477-1-AP; ProteinTech) was used at 1:1000 for Western blots and 1:200 for immunofluorescence. Anti-tom20 (sc-17764; Santa Cruz Biotechnology), anti-tubulin (11224-1-AP; ProteinTech), and anti-actin (60008-1-Ig, ProteinTech) were used at 1:5000 dilutions for Western blots. Anti-VPS13D (A304-691A; BETHY Laboratories) was used at 1:50 dilutions for coimmunoprecipitation and 1:100 for Western blots. The reagents NMS873 (TargetMol, T1853) and BODIPY 493/503 (Thermo Fisher, D3922) were used. Antibiotics such as G418 (10131027), puromycin (A1113803), hygromycin B (10687010), and blasticidin (A1113903) were obtained from Thermo Fisher (U.S.).
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2

Antibodies and Small Molecules Protocol

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Anti-HA (sc-7392, RRID:AB_627809) antibody was obtained from Santa Cruz, anti-Flag (F3165, RRID:AB_259529) antibody was obtained from Sigma, anti-SOX2 (23064, RRID:AB_2714146), anti-Nanog (8822, RRID:AB_11217637), anti-AKT (9272, RRID:AB_329827) and anti-SLC7A11 (12691, RRID:AB_2687474) antibodies were obtained from Cell Signaling Technology, anti-SLC7A11 (26864-1-AP, RRID:AB_2880661) for mouse, anti-Tubulin (11224-1-AP, RRID:AB_2210206), anti-Actin (60008-1-Ig, RRID:AB_2289225) and anti-GAPDH (60004-1-Ig, RRID: AB_2107436) antibodies were obtained from Proteintech, anti-Oct4 (ab18976, RRID:AB_444714) antibody was obtained from Abcam. Erastin (S7242), Imidazole ketone erastin (S8877), Fer-1 (S7243) and Cisplatin (S1166) were obtained from Selleck. MG132 (C2211), cycloheximide (CHX, C7698), PD146176 (P4620) and DFO (S1712) were obtained from Sigma.
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