The pulmonary tissue sections were incubated at 60 °C for 4 h and immersed in xylene twice for 20 min each, rehydrated by means of a graded series of ethanol, and then incubated with fresh 0.3% hydrogen peroxide in 100% methanol for 30 min at room temperature. The sections were then washed three times using PBS for 15 min. Antigen retrieval was performed with the ImmunoSaver antigen retriever system (Boster, AR0023, USA) at 98–100 °C for 30 min, and sections were cooled to room temperature. Sections were then washed by PBS. Nonspecific binging sites were blocked by incubation with BSA solution (Boster, SA1022, USA) for 30 min. The sections were then incubated with SFTPC antibody (Abclonal, A1835, USA) or Nanog antibody (Thermo Fisher Science, PA1-097, China) in a 1:100 dilution overnight at 4 °C and incubated with secondary antibodies at room temperature for 30 min. The sections were performed according to the steps of the SABC immunohistochemical staining kit (Boster, SA1022, USA). The sections were imaged using a × 40 objective.
Sftpc antibody
Manufactured by ABclonal
Sourced in United States, China
The SFTPC antibody is a laboratory reagent used for the detection and analysis of the SFTPC (Surfactant Protein C) protein. SFTPC is a critical component of the pulmonary surfactant system, which helps maintain the stability of the alveoli in the lungs. The SFTPC antibody can be used in various research applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to study the expression and localization of the SFTPC protein.
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Lab products found in correlation
Sabc immunohistochemical staining kit, by Boster Bio (1 mentions)
P21 antibody, by Abcam (1 mentions)
Nrf2 antibody, by Cell Signaling Technology (1 mentions)
P16 antibody, by Abcam (1 mentions)
Ki67 antibody, by Abways (1 mentions)
Pcna antibody, by Proteintech (1 mentions)
Keap1 antibody, by Proteintech (1 mentions)
Trim25 antibody, by Proteintech (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
2 protocols using sftpc antibody
1
Immunohistochemical Staining of Pulmonary Tissue
2
Immunofluorescence Staining of Cellular Proteins
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Tissue sections were deparaffinized and endogenous peroxidase was blocked with 3% H2O2. Sections were incubated in 5% albumin bovine V (BSA, Solarbio) for 1 h. The cells were washed three times with PBS and fixed with 4% paraformaldehyde for 15 min. After three washes with PBS, the cells were permeated with 0.1% Triton X-100 for 15 min. After three washes with PBS, the cells were incubated with 5% bovine serum albumin for 30 min at room temperature and then incubated with p21 antibody (1:300, Abcam, Cambridge, MA, USA), p16 antibody (1:200, Abcam), SFTPC antibody (1:200, Abclonal, Wuhan, China), Keap1 antibody (1:300, Proteintech, Wuhan, China), Nrf2 antibody (1:300, Cell Signaling Technology, USA), PCNA antibody (1:200, Proteintech), Ki67 antibody (1:200, Abways, Shanghai, China), or Trim25 antibody (1:200, Proteintech) at 4 °C overnight. The next day, after washing with PBS, fluorescein-labeled secondary antibodies (1:300, Abcam) for immunofluorescence were incubated. Nuclei were counterstained with DAPI (Invitrogen, USA). The samples were washed three times with PBS and photographed with fluorescence microscopy (Nikon, Japan).
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