Acquity lct premier xe

Fmoc (fluorenylmethyloxycarbonyl)-protected amino acids were purchased from Chem Impex. ChymoTrypsin (64.8 units/mg) was purchased from Sigma Aldrich. Trypsin (225 units/mg), papain (30.3 units/mg), and caspases 3 (100 units/µL) and 8 (0.2 mg/mL) were purchased from Fisher Scientific. All other chemicals were purchased from Sigma Aldrich. ¹H NMR and ¹³C NMR spectra were obtained on an Avance DRX 400 MHz instrument. ESI mass spectra were obtained using a Waters Acquity LCT Premier XE. Assay measurements were carried out on a Bio-Tek ELx 800 Microplate Reader.

NMR spectra were obtained on Bruker AV 500 and AV 600 MHz spectrometers with a relaxation delay of 4 seconds for both proton and carbon experiments. Infrared absorption spectra were obtained using a PerkinElmer FT-IR equipped with an attenuated total reflectance (ATR) accessory. High-resolution mass spectra were obtained for small molecules using a Waters Acquity LCT Premier XE equipped with an autosampler and direct injection port. High-resolution mass spectra and peptide mapping were acquired using a Thermo Q Exactive Plus Orbitrap Mass Spectrometer equipped with a direct injection port and switchable UltiMate 3000 nanoLC. Peptide masses were analyzed using Thermo-Fisher Proteome Discoverer software version 1.4. SDS-PAGE was performed using Bio-Rad Any kD Mini-PROTEAN-TGX gels and gels were stained with Coomassie for visualization of protein or 0.1 N iodine for visualization of PEG. SDS-PAGE protein standards were obtained from Bio-Rad (Precision Plus Protein Pre-stained Standards).