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Slide 2 well glass bottom chamber

Manufactured by Ibidi
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The Ibidi µ-slide 2-well glass bottom chamber is a laboratory equipment designed for cell culture and microscopy applications. It features a two-well configuration with a glass bottom, providing a suitable environment for cell adhesion and imaging.

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Lab products found in correlation

Low gelling agarose, by Merck Group (1 mentions)

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Chamber slides (62 citations) Glass chamber slides (8 citations) Glass slides (8 citations) Ibidi glass-bottom chambers (5 citations) Glass-bottom slides (2 citations)

2 protocols using slide 2 well glass bottom chamber

1

Embedding Larvae in Agarose for Live Imaging

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Larvae were embedded in 1 mL 1% low gelling agarose (Sigma-Aldrich) prepared in E3− in Ibidi µ-slide 2-well glass bottom chamber (Ibidi, Fitchburg, WI) and topped off with 1 mL E3−. Agarose and top-off solution were supplemented with 0.16 mg/mL tricaine to keep larvae anesthetized during live imaging. In drug treatment experiments, agarose and top-off solution were supplement with drugs at working concentrations.
Miskolci V., Tweed K.E., Lasarev M.R., Britt E.C., Walsh A.J., Zimmerman L.J., McDougal C.E., Cronan M.R., Fan J., Sauer J.D., Skala M.C, & Huttenlocher A. (2022). In vivo fluorescence lifetime imaging of macrophage intracellular metabolism during wound responses in zebrafish. eLife, 11, e66080.
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2

Larval Anesthesia and 2-DG Imaging

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Larvae were embedded in 1 mL 1% low gelling agarose prepared in E3 medium in Ibidi µ-Slide 2-Well Glass Bottom Chamber (Ibidi, Fitchburg, WI) and topped off with 1 mL E3 medium. Agarose and top-off solution were supplemented with 0.16 mg/mL Tricaine to keep larvae anesthetized during imaging. In 2-DG experiments, agarose and top-off solution were supplement with 5 mM 2-DG to maintain larvae in the inhibitor.
Miskolci V., Tweed K., Lasarev M., Britt E.C., McDougal C.E., Walsh A.J., Fan J., Sauer J., Skala M.C., & Huttenlocher A. (2020). In vivofluorescence lifetime imaging captures metabolic changes in macrophages during wound responses in zebrafish.
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