Plasma samples were collected under fasting conditions and twenty minutes following oral consumption of a mixed meal under deep anesthesia shortly prior euthanasia. Immediately after collection in tubes pretreated with a DPP-IV inhibitor (Merck), plasma was separated from the blood samples by centrifugation at 5 × 103 rpm for 10 min at 4 °C and stored at −80 °C. To measure GLP-1 and PYY levels (all samples in duplicates), rat-specific enzyme immunoassay/enzyme-linked immunosorbent (EIA/ELISA) kits were used (EK-028-11 and EK-059-04(Phoenix Pharmaceuticals)).
Ek 028 11
Manufactured by Phoenix Pharmaceuticals
Sourced in United States
The EK-028-11 is a compact and versatile laboratory centrifuge designed for a variety of applications. It features a high-speed motor and a sturdy construction to provide reliable and consistent performance. The centrifuge can accommodate a range of sample sizes and tube types, making it suitable for use in various laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using ek 028 11
1
Plasma Collection and GLP-1/PYY Quantification
2
Aortic Blood Sampling for GLP-1 and PYY
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples (pretreated with DPP-IV inhibitor (Merck)) were taken directly from the abdominal aorta at the end of the experimental period. Serum levels of GLP-1 (EK-028-11, Phoenix Pharmaceuticals, Burlingame, CA, USA) and PYY (EK-059-02, Phoenix Pharmaceuticals) were measured using ELISA. Glucose was measured with handheld measuring devices. Measurements of aspartate transaminase (AST) alanine transaminase (ALT), triglyceride (TG) and cholesterol (CHOL) were performed in an external laboratory (Laboklin, Bad Kissingen, Germany) with a cobas® 8000 modular analyzer (Roche Deutschland Holding GmbH).
3
Energy Drink Effects on Appetite Hormones
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All participants consumed 200 ml of an energy drink (Ensure Plus (Abbott, Chicago, IL, USA) or Resource® Energy chocolate (Nestlé, Vevey, Switzerland), identical in terms of kcal) after a period of fasting of 10 h. Blood samples were taken immediately before (0 min) and 15, 30, 45, and 60 min. Every blood sample, processed immediately after taking and pretreated with a Dipeptidylpeptidase-4 inhibitor (DPP4, Merck, Darmstadt, Germany) and aprotinin (Merck) was analyzed for concentrations of plasma/serum glucose, insulin, total PYY 3-36, total GLP-1, GLP-2, oxyntomodulin and leptin (Phoenix Pharmaceuticals (Burlingame, CA, USA), EK-028-11, EK-059-02, EK-003-12, EK-028-14, EK-028-22). GLP-2, oxyntomodulin and leptin were measured at 0 min and 30 min in groups CO-surgery and HO-surgery only. The participants rated their appetite using visual analogue scales (VAS) at the same time points and thereby stated hunger, satiety, abdominal fullness, and how much to still be able to eat of his/her favorite food. Levels of GLP-1/PYY were correlated with VAS ratings.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!