Millipore direct q3 uv purification system

l-Kynurenine sulfate, d-amphetamine sulfate and dizocilpine (5-methyl-10,11-dihydro-5H-dibenzo [a,d] cyclohepten-5,10-imine) were obtained from Sigma-Aldrich, USA. All drugs were dissolved in saline and prepared ex tempore. pH of KYN solution was adjusted to 7.0 with 0.1 N NaOH. D-amphetamine sulphate (1.0 mg/kg) and dizocilpine (doses 0.075, 0.15 and 0.3 mg/kg) were administered i.p. in a volume of 2 mL/kg. In all the cases, the control animals received corresponding saline injection. HPLC reagents were purchased from J.T. Baker Chemicals or Sigma-Aldrich. The analytical stable isotope-labeled standards of [²H₅]-tryptophan and [¹³C₆]-kynurenine were purchased from Alsachim (Illkirch-Graffenstaden, France). Acetonitrile and methanol for LC/MS analyses, of Optima® LC/MS grade, were purchased from Fisher Chemical (Waltham, MA, USA). Formic acid (LC/MS grade) and diethyl ether (HPLC grade) were purchased from Merck KGaA (Darmstadt, Germany). Titan Syringe Filters RC, 0.2 μm were supplied by Thermo Scientific (Waltham, MA, USA). The ultra-pure water was obtained from Millipore Direct-Q3-UV purification system (Merck KGaA, Germany).

The high purity (>98%) analytical standards of tetraconanazole, fluopyram and diazinon were purchased from Merck KGaA (Darmstadt, Germany). Stock solution of each pesticide at a concentration of 1000 mg L^-1 were prepared in acetonitrile and stored at 4°C. The working multicompund standard solutions for matrix-matched calibrations were prepared by appropriate dilution of the stock solutions with acetonitrile. Acetonitrile and methanol (both Optima^® LC-MS grade) were pourchased from Fischer Chemical (Waltham, MA, USA). Formic acid (LC-MS grade) and triphenyl phosphate (TPP) were obtained from Merck KGaA (Darmstadt, Germany). Agilent Bond Elut QuEChERS EN Method (buffer salt mixture, polypropylene (PP) tubes and sorbents for dispersive—SPE (dispersive solid phase extraction) including 50 mg primary secondary amine (PSA), 50 mg C18, and 50 mg graphitized carbon black (GCB)) were purchased from Agilent Technologies (Santa Clara, CA, USA). The ultra-pure water was obtained from Millipore Direct-Q3- UV purification system (Merck KGaA, Germany).

Antihypertensive drugs: ramipril, lercanidipine, valsartan, hydrochlorothiazide, telmisartan, perindopril, and nebivolol were purchased from Sigma-Aldrich (St. Louis, MO, USA). The structures, physicochemical properties and drug classes (pharmacological properties) of investigated substances are presented in Table 5. Chromatographic glass plates, HPTLC silica gel 60 F254 and HPTLC diol F254 were supplied by Merck (Darmstadt, Germany). Acetonitrile and methanol (both MS purity) were purchased from Fisher Chemical (Waltham, MA, USA). Ethanol, toluene and formic acid (98–100%) LC-MS grades were supplied by Merck (Darmstadt, Germany). The ultra-pure water was obtained from the Millipore Direct-Q3-UV purification system (Merck, Darmstadt, Germany). The plasma samples (also a reference sample) were kindly provided by the Cardiology Clinic of the Independent Public Clinical Hospital No. 4 in Lublin (Lublin, Poland).