FKA (T3S0737, purity: 99.41%) and 3-Methyladenine (3 MA) were acquired from TOPSCIENCE (Shanghai, China), and the mouse IL-1β cytokine was obtained from the R&D system (501-RL-010, United States). Safranin O solution were provided by Solarbio (Beijing, China). The primary antibodies for iNOS, COX2, Col2, Aggrecan, Sox9, and immunofluorescence secondary antibodies were acquired from Abcam (Shanghai, China). Atg12, Beclin, LC3I/II, P21, P16, Bax, Bcl2, GAPDH, and antibodies for all pathways were purchased from CST (Beverly, MA, United States). Corresponding primary antibodies for MMP3/13 and Nrf2/HO-1/NQO1 were supplied by the Proteintech Group (Wuhan, Hubei, China). ADAMTS5 primary antibody, secondary antibody, phosphate-buffered saline (PBS), trypsin, collagenase type II, the CCK8 assay kit, bovine serum albumin (BSA), and protein extraction kit were ordered and acquired from Boster Biological Technology (Wuhan, Hubei, China). RFP-GFP-LC3-adenovirus was purchased from Hanbio (Shanghai, China). The Senescence β-galactosidase staining kit and the Annexin V-FITC apoptosis detection kit were supplied by Beyotime (Shanghai, China).
3 methyladenine 3 ma
Manufactured by Topscience
Sourced in China
3-Methyladenine (3 MA) is a chemical compound that serves as an inhibitor of autophagy, a cellular process involving the degradation and recycling of damaged or unwanted cellular components. This compound acts by blocking the formation of autophagosomes, which are the vesicles responsible for transporting cellular material to the lysosome for degradation. 3-Methyladenine is commonly used in research applications to study the role of autophagy in various biological processes and disease states.
Automatically generated - may contain errors
Lab products found in correlation
Protein extraction kit, by Boster Bio (1 mentions)
Trypsin, by Boster Bio (1 mentions)
Phosphate buffered saline (pbs), by Boster Bio (1 mentions)
Bovine serum albumin (bsa), by Boster Bio (1 mentions)
Cck 8 assay kit, by Boster Bio (1 mentions)
Collagenase type 2, by Boster Bio (1 mentions)
Rfp gfp lc3 adenovirus, by Hanbio Biotechnology (1 mentions)
Safranin o solution, by Solarbio (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Beyotime (1 mentions)
Senescence β galactosidase staining kit, by Beyotime (1 mentions)
Rapamycin rapa, by AbMole (1 mentions)
2 protocols using 3 methyladenine 3 ma
1
Chondrocyte Senescence and Apoptosis
2
Autophagy modulation in neuronal cells
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PC12 cells (RRID: CVCL_F659), which were commonly adopted in neurological disease research because of their neurogenicity, differentiability, high autophagic activity, and ease of experimental manipulation, were purchased from the Kunming Cell Bank of the Chinese Academy of Sciences (Cat# KCB93033YJ). U87-GFP-LC3 cells with stable GFP-LC3II expression (Macau University of Science and Technology, Macao SAR, China), a common cell line used to study autophagy, were cultured in DMEM containing 10% (v/v) fetal bovine serum and 0.1% penicillin/streptomycin in a humidified 5% CO2 atmosphere at 37°C. CRSE was used in cells at concentrations of 20, 40, and 80 μg/mL, rapamycin (Rapa; Cat# M1768, AbMole, Shanghai, China) at 10 μM and 3-methyladenine (3-MA; Cat# T1879, Topscience, Shanghai, China) at 2 μM. The duration of drug intervention was 24 hours under normal conditions.
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