The largest database of trusted experimental protocols

2 protocols using phospho p70 s6 kinase thr389 9234

1

Astragaloside IV Modulates Immune Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Astragaloside IV (purity > 98%) was purchased from Ronghe Co. (Shanghai, China). OVA (purity > 98%, Grade V) and methacholine (purity > 98%) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Imject alum adjuvant was obtained from Thermo Fisher Scientific Co. (Waltham, MA, USA). Dexamethasone sodium phosphate injection (purity > 99%; 5 mg/mL) was provided by Tianjin pharmaceutical group Xinzheng Co. (Tianjin, China). Rapamycin (purity > 98%) was purchased from LC Laboratories (Woburn, MA, USA). ELISA kits for INF-γ, IL-4, IL-5, IL-13, and IL-17A, FITC-labeled anti-rat CD4, APC-labeled anti-rat CD25, and PE-labeled anti-rat Foxp3 were purchased from eBioscience Co. (San Diego, USA). Antibodies for phospho-S6 ribosomal protein (Ser235/236) #4858, phospho-Akt (Ser473) #4060, Akt (#4691), phospho-4E-BP1 (Ser65) #9451, phospho-p70 S6 Kinase (Thr389) #9234, and β-actin were obtained from Cell Signaling (Danvers, MA, USA).
+ Open protocol
+ Expand
2

Western Blotting Antibody Dilutions

Check if the same lab product or an alternative is used in the 5 most similar protocols
All antibodies were used at a dilution of 1:1,000–3,000 in TBST buffer with 5% non-fat milk for Western blotting. Antibodies to mTOR (2932), GβL (3274), Sin1 (12860), phospho-AKT Ser473 (4060), phospho-AKT Thr308 (13038), AKT (pan; 4691), Rictor (2114), phospho-p70 S6 kinase Thr389 (9234), p70 S6 kinase (9202), PDK1 (13037), histone H3 (4499), phospho-PKC (pan; 9371), PKCζ (9368), cleaved PARP (5625), DNA-PKcs (4602), phospho-ATM/ATR substrate (2851), phospho-p53 Ser15 (9286), GST (2622), rabbit antibody to HA (3724) and Myc-tag (2278), and mouse antibody to Myc-tag (2276) were purchased from Cell Signaling Technology. Mouse monoclonal antibody to HA (901503) was purchased from BioLegend. Antibodies to mouse IgG (A4416), rabbit IgG (A4914), rabbit-anti-Flag (F7425) and mouse-anti-Flag (F3165) were purchased from Sigma Aldrich. Antibodies to α-tubulin (66031–1-Ig) and PARP1 (13371–1-AP) were purchased from Proteintech. Antibodies to ECT2 (sc-1005, discontinued) and LIG4 (sc-28232, discontinued) were purchased from Santa Cruz Biotechnology. Antibody to BRCA1 (MABC 199) was purchased from EMD Millipore. Antibody to 53BP1 (612523) was purchased from BD Biosciences.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!