Pe conjugated anti siglec f e50 2440

Manufactured by BD

Sourced in United States

The PE-conjugated anti-Siglec-F (E50-2440) is a monoclonal antibody that binds to the Siglec-F receptor. Siglec-F is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family and is expressed on eosinophils and a subset of alveolar macrophages. The PE (phycoerythrin) fluorescent label allows for detection and analysis of Siglec-F-expressing cells using flow cytometry.

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Lab products found in correlation

Z yvad fmk, by Abcam (1 mentions) Glibenclamide, by Merck Group (1 mentions) Apocynin, by Merck Group (1 mentions) Ca 074 methyl ester, by Merck Group (1 mentions) Apc conjugated anti cd11c n418, by Thermo Fisher Scientific (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Sb203580, by Merck Group (1 mentions) N acetyl l cysteine, by Merck Group (1 mentions) U1026, by Merck Group (1 mentions) Live dead fixable red, by Thermo Fisher Scientific (1 mentions) Bapta am, by Merck Group (1 mentions) Recombinant mouse il 3, by BioLegend (1 mentions) Anti nk1.1 pk136, by BD (1 mentions) Il 33, by BioLegend (1 mentions) Apc cy7 conjugated streptavidin, by BioLegend (1 mentions) Anti tubulin 2144, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Siglec-F (101 citations) E50-2440 (19 citations) PE-conjugated anti-Siglec-F (13 citations) PE anti-Siglec-F (5 citations) PE)–anti-Siglec-F (E50-2440; (2 citations)

3 protocols using pe conjugated anti siglec f e50 2440

Dectin-1, Dectin-2 and CR3 Signaling Inhibition

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Blocking antibodies anti-Dectin-1 (2A11), anti-Dectin-2 (D2.11E4) and anti-CR3 (5C6) were purchased from Serotec and anti-TLR-2 (6C2), IgG2a (eBR2a) and IgG2b (eB149/10H5) were from eBioscience. PE/Cy7-conjugated anti-CD45 (30-F11), APC-conjugated anti-F4/80 (BM8), Alexa 488-conjugated anti-CD103 (2E7) and Alexa 647-conjugated anti-Ly6G (1A8) were obtained from BioLegend. APC-conjugated anti-CD11c (N418) and PE-conjugated anti-CD11b (M1/70) were from eBioscience and PE-conjugated anti-Siglec-F (E50-2440) from Pharmingen.
Z-YVAD-FMK (Caspase-1 inhibitor) was obtained from BioVision. U1026 (ERK inhibitor), SP600125 (JNK inhibitor), SB203580 (p38 inhibitor), N-acetyl-L-cysteine (ROS scavenger), Apocynin (NADPH-oxidase inhibitor), Ca-074 methyl ester (cathepsin B inhibitor), bafilomycin A1 (phagosomal acidification inhibitor) and glibenclamide (K⁺ channel inhibitor) were obtained from Sigma-Aldrich.

Chang T.H., Huang J.H., Lin H.C., Chen W.Y., Lee Y.H., Hsu L.C., Netea M.G., Ting J.P, & Wu-Hsieh B.A. (2017). Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum. PLoS Pathogens, 13(7), e1006485.

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Comprehensive Immune Cell Profiling in BALF

Cited 1 time

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The staining panel for immune cell subsets in BALF was designed according to previous studies [44 (link),45 (link)]. Briefly, the BALF cells were stained with fluorochrome-conjugated monoclonal antibodies at 4 °C for 30 min. The antibodies included FITC conjugated anti-Ly6G (1A8; BD Biosciences, San Jose, CA, USA), PE-conjugated anti-Siglec-F (E50-2440; BD Biosciences), APC-conjugated anti-B220 (RA3-6B2; BD Biosciences), APC-conjugated anti-CD3 (145-2C11; BD Biosciences), PerCP/Cy5.5-conjugated anti-CD11b (M1/70; BioLegend, San Diego, CA, USA), eFluor 450-conjugated anti-CD11c (N41B; Invitrogen, Eugene, OR, USA), and Live/Dead fixable Red (Invitrogen). After washing, the samples were analyzed with multi-parametric flow cytometry (LSR II; BD Boisciences) and data were analyzed with FlowJo software (version 10, Tree Star, Inc., Ashland, OR, USA).

Chen I.C., Wang S.C., Chen Y.T., Tseng H.H., Liu P.L., Lin T.C., Wu H.E., Chen Y.R., Tseng Y.H., Hsu J.H., Dai Z.K., Suen J.L, & Li C.Y. (2021). Corylin Ameliorates LPS-Induced Acute Lung Injury via Suppressing the MAPKs and IL-6/STAT3 Signaling Pathways. Pharmaceuticals, 14(10), 1046.

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Identification of Immune Cell Populations

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The following Abs were purchased from BD Pharmingen: allophycocyanin (APC)-conjugated anti-CD49b (HM2), phycoerythrin (PE)-conjugated anti-Siglec-F (E50-2440), and anti-NK1.1 (PK136). Unlabeled anti-IL-3 (MP2-8F8) and rat IgG1 isotype (RTK2071); biotin-conjugated anti-CD49b (DX5), anti-CD123 (IL-3R, 5B11), anti-FcRI (MAR-1), anti-I-A/I-E (M5/114.15.2), American hamster IgG isotype (HTK888), and rat IgG2a isotype (RTK2758); PE-conjugated CD200R3 (Ba13), anti-IgE (RME-1), and anti-CD4 (RM4.5); APC-conjugated anti-Gr-1 (RB6-8C5), anti-B220 (RA3-6B2), and anti-F4/80 (BM8); PE/Cy7-conjugated anti-c-kit (2B8) and anti-CD45 (30-F11); Brilliant Violet 421-conjugated anti-CD3 (145-2C11) and anti-CD11c (N418); and APC/Cy7-conjugated streptavidin were from BioLegend. PE-conjugated CD11b Abs (M1/70) were obtained from eBioscience. Anti-STIM1 (D88E10), anti-STIM2 (4917), and anti--tubulin (2144) were from Cell Signaling Technology. TNP-specific IgE (IGEL-b4) and anti-CD16/32 (2.4G2) were prepared in our laboratory. TAM (T5648), DT (D0564), inactive mDT (D2189), EGTA, BAPTA-AM, and DPI were purchased from Sigma-Aldrich. Recombinant mouse IL-3 and IL-33 were obtained from BioLegend.

Yoshikawa S., Oh-Hora M., Hashimoto R., Nagao T., Peters L., Egawa M., Ohta T., Miyake K., Adachi T., Kawano Y., Yamanishi Y, & Karasuyama H. (2019). Pivotal role of STIM2, but not STIM1, in IL-4 production by IL-3-stimulated murine basophils. Science signaling, 12(576).

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