Log In Sign up
The largest database of trusted experimental protocols

Rat anti ft

Manufactured by Merck Group
Visit Supplier

The Rat anti-Ft is a laboratory equipment used for immunological research. It functions as a reagent for the detection and analysis of specific protein targets in biological samples. This product provides a standardized and reliable tool for researchers to investigate cellular processes and signaling pathways.

Automatically generated - may contain errors

Lab products found in correlation

Mouse anti v5, by Thermo Fisher Scientific (2 mentions) R960 25, by Thermo Fisher Scientific (2 mentions) Mouse anti actin, by Merck Group (2 mentions) Mouse anti flag, by Merck Group (2 mentions) Mouse anti biotin, by Santa Cruz Biotechnology (2 mentions) Mouse anti gst, by Santa Cruz Biotechnology (2 mentions)

2 protocols using rat anti ft

1

Immunoblotting Antibody Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysates were analysed by standard chemiluminescent immunoblotting techniques. Primary antibodies: mouse anti-actin 1:2000 (C-4; Sigma Aldrich), mouse anti-Biotin 1:400 (33; Santa Cruz Biotechnology), rat anti-Ft 1:500 (Sopko et al., 2009 (link)), mouse anti-FLAG 1:5000 (M2; Sigma Aldrich), mouse anti-GST 1:500 (B-14; Santa Cruz Biotechnology), rabbit anti-HA 1:1000 (C29F4; Cell Signaling Technology), rat anti-HA 1:2000 (3F10; Roche Applied Science) and mouse anti-V5 1:5000 (R960-25; Thermo Fisher Scientific).
Fulford A.D., Enderle L., Rusch J., Hodzic D., Holder M.V., Earl A., Oh R.H., Tapon N, & McNeill H. (2023). Expanded directly binds conserved regions of Fat to restrain growth via the Hippo pathway. The Journal of Cell Biology, 222(5), e202204059.
+ Open protocol
+ Expand
2

Immunoblotting Antibody Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysates were analysed by standard chemiluminescent immunoblotting techniques. Primary antibodies: mouse anti-actin 1:2000 (C-4; Sigma Aldrich), mouse anti-Biotin 1:400 (33; Santa Cruz Biotechnology), rat anti-Ft 1:500 (Sopko et al., 2009 (link)), mouse anti-FLAG 1:5000 (M2; Sigma Aldrich), mouse anti-GST 1:500 (B-14; Santa Cruz Biotechnology), rabbit anti-HA 1:1000 (C29F4; Cell Signaling Technology), rat anti-HA 1:2000 (3F10; Roche Applied Science) and mouse anti-V5 1:5000 (R960-25; Thermo Fisher Scientific).
Käck H., Gibson K.J., Lindqvist Y, & Schneider G. (1998). Snapshot of a phosphorylated substrate intermediate by kinetic crystallography. Proceedings of the National Academy of Sciences of the United States of America, 95(10), 5495-5500.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!