Cereplex system

LFP, electromyogram (EMG), and accelerometer (ACC) signals and video were monitored continuously from video-EEG device for offline processing. Intracranial electrode signals were fed through a high input impedance, DC-cut at 1 Hz, gain of 1000, 16-channel amplifier and digitized at 20 kHz (Xcell, Dipsi, Cancale, France) for half of the recordings and through a Blackrock Cereplex System using the Cereplex Direct Software Suite (version 7.0.6.0) developed by Blackrock Microsystems (Salt Lake City, UT, USA) for the second half, together with a synchronization signal from the ultrasound scanner. LFP signals were pre-amplified and digitized onto the animal’s head which prevent artifacts originating from cable movement. Custom-made software based on LabVIEW 2016 (National Instruments, Austin, TX, USA) simultaneously acquired video from a camera pointed at the recording stage. A regular amplifier was used, and no additional electronic circuit for artifact suppression was necessary. A large bandwidth amplifier was used, which can record local field potentials in all physiological bands (LFP, 0.1–2 kHz). The spatial resolution of LFPs ranges from 250 µm to a few mm radius.

LFP, electromyogram (EMG) and accelerometer (ACC) signals and video were monitored continuously from video-EEG device for offline processing. Intracranial electrode signals were fed through a Blackrock Cereplex System using the Cereplex Direct Software Suite (version 7.0.6.0) developed by Blackrock Microsystems (Salt Lake City, UT, USA), together with a synchronization signal from the ultrasound scanner. LFP signals were pre-amplified and digitized onto the animal’s head which prevent artifacts originating from cable movement. A regular amplifier was used, and no additional electronic circuit for artifact suppression was necessary. A large bandwidth amplifier was used, which can record local field potentials in all physiological bands (LFP, 0.1–2 kHz). The spatial resolution of LFPs ranges from 250 µm to a few mm radius.