Gpo trinder triglyceride assay kit

Manufactured by Applygen

Sourced in China

The GPO-Trinder triglyceride assay kit is a laboratory equipment product designed to measure the concentration of triglycerides in biological samples. It utilizes the GPO-Trinder enzymatic method to quantify triglyceride levels.

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Lab products found in correlation

Microtiter plate reader, by Agilent Technologies (4 mentions) Bca protein assay, by Thermo Fisher Scientific (2 mentions) Hp 88 column, by Agilent Technologies (1 mentions) Gas chromatography mass spectrometer, by Agilent Technologies (1 mentions) Microtiter plate reader, by Bio-Rad (1 mentions)

7 protocols using gpo trinder triglyceride assay kit

Lipid Profiling in Bovine Mammary Cells

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Total cellular triacylglycerol (TAG) was extracted according to the GPO-Trinder triglyceride assay kit protocol (Applygen Technologies, Shanghai, China). The mass of TAG was determined according to the manufacturer's instructions on a micro-titer plate reader (BioTek Instruments, Inc., VT, USA). Quantification of total cellular TAG was normalized to protein concentration determined in each well using a BCA protein assay (Thermofisher, Waltham, MA, USA) according to the manufacturer's instructions.
Oil Red O staining was performed on GMEC according to a method described previously [37 (link)]. The images were captured using a Leica fluorescent microscope (DMI4000B, Weztlar, Germany).
For assay of FA profiles, collected GMEC were scraped off the culture dish using a 2-mL aliquot of 2.5% (vol/vol) vitriol:methanol. Then, total lipid extraction and methylation were performed according to Shi et al. [38 (link)]. Methylated lipid samples were analyzed using a Gas Chromatography-Mass Spectrometer (Agilent Technologies, Santa Clara, CA, USA) installed with an HP-88 column (100 m × 0.25 mm i.d. × 0.25 μm film thickness, Agilent Technologies) following a published procedure [8 (link)]. The relative proportion of each FA was determined as the ratio of the FA peak to the total peaks in each run. Data for each FA were analyzed as a proportion of the total FAs detected.

Zhang W., Zhang C., Luo J., Xu H., Liu J., Loor J.J, & Shi H. (2022). The LXRB-SREBP1 network regulates lipogenic homeostasis by controlling the synthesis of polyunsaturated fatty acids in goat mammary epithelial cells. Journal of Animal Science and Biotechnology, 13, 120.

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Quantification of Intracellular Triglycerides

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After 48 h of incubation with 0 or 1 μM T0901317, cells were rinsed 3 times with PBS and then harvested in lysis buffer (50 mM Tris-HCL, pH 7.4, 150 mM NaCl, 1% Triton X-100), and cells were scraped off the culture dish and sonicated to homogenize the cell suspension. Intracellular total TAG was measured according to the GPO-Trinder Triglyceride Assay Kit (Applygen Technologies Inc., Beijing, China). The concentration of TAG was determined on a microtiter plate reader (Bio-Rad) and normalized to total cellular protein assessed using a BCA protein assay (Thermo Fisher Scientific) according to the manufacturer's instructions (Kang et al., 2015) .

Xu H.F., Luo J., Zhang X.Y., Li J, & Bionaz M. (2019). Activation of liver X receptor promotes fatty acid synthesis in goat mammary epithelial cells via modulation of SREBP1 expression. Journal of dairy science, 102(4).

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Cellular Triglyceride Quantification Assay

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The protocol for cellular TAG assay was described previously (Kang et al., 2015; Shi et al., 2015b) . Briefly, total cellular TAG was extracted using the GPO-Trinder triglyceride assay kit (http:// www .applygen .com/ a/ meixueyushenghuaceding/ 276 .html; Applygen Technologies, Beijing, China). The concentration of TAG was determined according to the manufacturer's instructions on a plate reader (Thermo Fisher Scientific). The concentrations were calculated using the equation obtained from a linear regression of the standard curve, which was established according to the manufacturer's procedure (http:// www .applygen .com/ a/ meixueyushenghuaceding/ 276 .html). The quantification of total cellular TAG was normalized to the cellular protein concentration. Protein concentration of each well was determined using a BCA protein assay kit (Pierce, Thermo Fisher Scientific) according to the manufacturer's instructions (https:// www .thermofisher .com/ order/ catalog/ product/ 23225).

Shi H.B., Du Y., Zhang C.H., Sun C., He Y.L., Wu Y.H., Liu J.X., Luo J, & Loor J.J. (2018). Fatty acid elongase 5 (ELOVL5) alters the synthesis of long-chain unsaturated fatty acids in goat mammary epithelial cells. Journal of dairy science, 101(5).

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Quantification of Cellular Triglycerides

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Total cellular TG was extracted according to the protocol of the GPO-Trinder triglyceride assay kit (Applygen Technologies, Beijing, China). Briefly, cells were collected and suspended in lysis buffer, following by sonication for 20 s. After centrifuging the cell suspension at 10,000 × g for 10 min at 4°C, the supernatant was removed for TG and protein analysis. The lysis buffer was used as blank control to correct the values of samples and standards. The concentration of TG was determined according to the manufacturer's instructions on a microtiter plate reader (BioTek, Winooski, VT). The concentrations were calculated using the equation obtained from linear regression of the standard curve. The quantification of total cellular TG was normalized to the cellular protein concentration. Protein concentration of each well was determined using a BCA protein assay (Pierce) according to the manufacturer's instructions.

Shi H.B., Yu K., Luo J., Li J., Tian H.B., Zhu J.J., Sun Y.T., Yao D.W., Xu H.F., Shi H.P, & Loor J.J. (2015). Adipocyte differentiation-related protein promotes lipid accumulation in goat mammary epithelial cells. Journal of dairy science, 98(10).

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Quantitative Cellular Triglyceride Assay

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The protocol for cellular TAG assay was described previously (Kang et al., 2015) . Briefly, total cellular TAG was extracted using the GPO-Trinder triglyceride assay kit (http://www.applygen.com/a/meixueyushen-ghuaceding/276.html; Applygen Technologies, Beijing, China). The concentration of TAG was determined according to the manufacturer's instructions on a plate reader (Thermo Fisher Scientific, Waltham, MA). The concentrations were calculated using the equation obtained from a linear regression of the standard curve which was established according to the manufacturer's procedure (http://www.applygen.com/a/meixueyush-enghuaceding/276.html). The quantification of total cellular TAG was normalized to the cellular protein concentration. Protein concentration of each well was determined using a BCA protein assay kit (Pierce, Thermo Fisher Scientific) according to the manufacturer's instructions (https://www.thermofisher.com/ order/catalog/product/23225).

Shi H.B., Wu M., Zhu J.J., Zhang C.H., Yao D.W., Luo J, & Loor J.J. (2017). Fatty acid elongase 6 plays a role in the synthesis of long-chain fatty acids in goat mammary epithelial cells. Journal of dairy science, 100(6).

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Quantification of Cellular Triglycerides

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Total cellular TAG was extracted according to the protocol of the GPO-Trinder triglyceride assay kit (Applygen Technologies, Beijing, China; http://www. applygen.com/a/meixueyushenghuaceding/276.html). The concentration of TAG was determined according to the manufacturer's instructions on a microtiter plate reader (BioTek, Winooski, VT). We calculated the concentrations using the equation obtained from linear regression of the standard curve. The quantification of total cellular TAG was normalized to the cellular protein concentration. We determined the protein concentration for each well using a bicinchoninic protein assay (Pierce; Thermo Fisher Scientific, Waltham, MA) according to the manufacturer's instructions.

Shi H.B., Zhang C.H., Zhao W., Luo J, & Loor J.J. (2017). Peroxisome proliferator-activated receptor delta facilitates lipid secretion and catabolism of fatty acids in dairy goat mammary epithelial cells. Journal of dairy science, 100(1).

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Quantification of Cellular Triglycerides

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Total cellular TAG was extracted according to the protocol of the GPO-Trinder triglyceride assay kit (Applygen Technologies, Beijing, China). The concentration of TAG was determined according to the manufacturer's instructions on a microtiter plate reader (BioTek, Winooski, VT). The concentrations were calculated using the equation obtained from linear regression of the standard curve. The quantification of total cellular TAG was normalized to the cellular protein concentration. Protein concentration of each well was determined using a BCA protein assay (Pierce, Thermo Fisher Scientific) according to the manufacturer's instructions (Shi et al., 2017) .

Shi H.B., Zhang C.H., Xu Z.A., Lou G.G., Liu J.X., Luo J, & Loor J.J. (2018). Peroxisome proliferator-activated receptor delta regulates lipid droplet formation and transport in goat mammary epithelial cells. Journal of dairy science, 101(3).

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