A permanent mouse fibroblast cell line, BALB/3T3 clone A31 cells (JCRB Cell Bank, Tokyo, Japan, JCRB9005). and a primary human keratinocyte cell line, NHEK-Ad cells (Lonza Japan Ltd., Tokyo, Japan) were cultured in DMEM (Thermo Fisher Scientific) supplemented with FBS (10%), 100 U/mL penicillin (Meiji Seika Pharma Co. Tokyo, Japan), and 100 μg/mL streptomycin (Meiji Seika Pharma) [27 (link),28 (link)] and in KBM-Gold medium (Lonza Japan), respectively. In total, 1 × 104 cells were added to each well of a 96-well Nunc MicroWell microplate (Thermo Fisher Scientific) before being cultured for 24 h for BALB/3T3 and 48 h for NHEK-Ad cells in a humidified atmosphere containing 5% CO2 at 37 °C [29 (link)].
Nhek ad cells
Manufactured by Lonza
Sourced in Japan
NHEK-Ad cells are normal human epidermal keratinocytes that have been immortalized through adenoviral transduction. These cells are suitable for use in various in vitro cell-based assays and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Kbm gold medium, by Lonza (1 mentions)
Penicillin, by Meiji Seika Pharma (1 mentions)
Streptomycin, by Meiji Seika Pharma (1 mentions)
Nunc microwell 96 well microplate, by Thermo Fisher Scientific (1 mentions)
Kgm gold bulletkit medium, by Lonza (1 mentions)
Fgm 2 bulletkit medium, by Lonza (1 mentions)
2 protocols using nhek ad cells
1
Cell Culture of BALB/3T3 and NHEK-Ad Lines
2
In Vitro Fibroblast and Keratinocyte Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Adult normal human dermal fibroblasts, NHDF‐Ad cells (Lonza Japan Ltd, Tokyo, Japan, CC‐2511), and adult normal human epidermal keratinocytes, NHEK‐Ad cells (Lonza Japan Ltd, 00192627), were cultured in FGM™ − 2 BulletKit™ medium (Lonza Japan Ltd)12 and KGM‐Gold™ BulletKit™ medium (Lonza Japan Ltd),13 respectively. In total, 1 × 104 NHDF‐Ad cells were added to each well of Nunc™ MicroWell™ 96‐well microplates (Thermo Fisher Scientific K. K., Tokyo, Japan) before being pre‐cultured for 24 hours in the cytotoxicity assay. In total, 2 × 105 NHEK‐Ad cells were added to each well of Nunc™ MicroWell™ 12‐well microplates (Thermo Fisher Scientific K. K.) before being pre‐cultured for 20 hours in the scratch assay and for 24 hours in the live/dead assay. All cells were cultured in a humidified atmosphere containing 5% CO2 at 37°C.
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