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Red fluorescent propidium iodide

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Propidium iodide is a fluorescent dye that binds to DNA. It is commonly used in flow cytometry and microscopy applications to stain and detect nucleic acids.

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Lab products found in correlation

Cellevent caspase 3 7 green detection reagent, by Thermo Fisher Scientific (6 mentions) Celltrace calcein green am, by Thermo Fisher Scientific (2 mentions) Viewlux μhts microplate imager, by PerkinElmer (2 mentions) Incucyte zoom live cell analysis system, by Sartorius (2 mentions) Celltiter glo 3d, by Promega (2 mentions) Celltiter glo, by Promega (2 mentions) Novocyte 3000, by Agilent Technologies (1 mentions)

3 protocols using red fluorescent propidium iodide

1

Apoptosis Induction in HepG2 Cells

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HepG2 cells were seeded in a 6-well plate (8 × 105 cells/well) and they were treated with YVVNPDNDEN (350 μM), YVVNPDNNEN (350 μM) or simvastatin (20 μM). After 24 h, cells were washed with phosphate-buffered saline (PBS), centrifuged and stained with FITC annexin V and red-fluorescent propidium iodide (Thermo Fisher). All the samples were analysed by flow cytometry (Novocyte 3000, ACEA Bioscience, San Diego, CA, USA), measuring the fluorescence emission at 530 nm and >575 nm.
Macchi C., Greco M.F., Ferri N., Magni P., Arnoldi A., Corsini A., Sirtori C.R., Ruscica M, & Lammi C. (2021). Impact of Soy β-Conglycinin Peptides on PCSK9 Protein Expression in HepG2 Cells. Nutrients, 14(1), 193.
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2

Multiparametric Cell Viability Assay

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Simultaneous staining with CellTrace calcein green AM (Thermo Fisher Scientific, C34852, 1:2000) to indicate intracellular esterase activity and red fluorescent propidium iodide (Thermo Fisher Scientific, P3566, 1:2000) to indicate loss of plasma membrane integrity allowed quick discrimination between live and dead cells. Caspase-3/7 activation in live cells was monitored using CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific, C10423, 1:1000) in IncuCyte Zoom Live Cell Analysis System (Sartorius). Cells were plated at a density of 106 cells/well in 6-well plates, treated with indicated compounds and imaged with a 10x objective. The CellTiter-Glo and CellTiter-Glo 3D assays (Promega, G9241, G9681) were carried out following the manufacturer’s instructions to quantify cell viability in adherent and EB cultures, respectively. Luminescence signal was read using the ViewLux μHTS microplate imager (Perkin Elmer).
Chen Y., Tristan C.A., Chen L., Jovanovic V.M., Malley C., Chu P.H., Ryu S., Deng T., Ormanoglu P., Tao D., Fang Y., Slamecka J., Hong H., LeClair C.A., Michael S., Austin C.P., Simeonov A, & Singeç I. (2021). A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells. Nature methods, 18(5), 528-541.
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3

Multiparametric Cell Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Simultaneous staining with CellTrace calcein green AM (Thermo Fisher Scientific, C34852, 1:2000) to indicate intracellular esterase activity and red fluorescent propidium iodide (Thermo Fisher Scientific, P3566, 1:2000) to indicate loss of plasma membrane integrity allowed quick discrimination between live and dead cells. Caspase-3/7 activation in live cells was monitored using CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific, C10423, 1:1000) in IncuCyte Zoom Live Cell Analysis System (Sartorius). Cells were plated at a density of 106 cells/well in 6-well plates, treated with indicated compounds and imaged with a 10x objective. The CellTiter-Glo and CellTiter-Glo 3D assays (Promega, G9241, G9681) were carried out following the manufacturer’s instructions to quantify cell viability in adherent and EB cultures, respectively. Luminescence signal was read using the ViewLux μHTS microplate imager (Perkin Elmer).
Chen Y., Tristan C.A., Chen L., Jovanovic V.M., Malley C., Chu P.H., Ryu S., Deng T., Ormanoglu P., Tao D., Fang Y., Slamecka J., Hong H., LeClair C.A., Michael S., Austin C.P., Simeonov A, & Singeç I. (2021). A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells. Nature methods, 18(5), 528-541.
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