Sodium cyanide

Human Grx1 (IMCO Corporation Ltd AB, Stockholm, Sweden) and human Grx2 (IMCO Corporation Ltd AB, Stockholm, Sweden) were adjusted to 1 mM each using 20 mM potassium phosphate buffer, pH 7.2, as a stock solution. E. coli Grx1 (IMCO Corporation Ltd AB, Stockholm, Sweden) and E. coli mutant Grx (C14S, Cys¹⁴ of E. coli Grx1 was replaced with Ser) (IMCO Corporation Ltd AB, Stockholm, Sweden) were adjusted to 3 mM each with 20 mM potassium phosphate buffer, pH 7.2. Human GRD (Sigma-Aldrich, Inc. St. Louis, MO, USA) and E. coli GRD (Novus Biologicals, Centennial, CO, USA) were 304 μM and 19.531 μM, respectively, in their original solutions. NADPH (Sigma-Aldrich, Inc. MO, USA) and GSH (Sigma-Aldrich, Inc.) were adjusted to 10 mM each with potassium phosphate buffer, pH 7.2. Sodium thiosulfate, sodium cyanide, and other chemicals were purchased from Wako Pure Chemicals Industries, Ltd. (Osaka, Japan).

Fe^IIITPPS (F), Py3CD (P), and Im3CD (I) were synthesized in our laboratory. The synthetic procedures were previously reported (46 (link), 48 , 70 (link)). Sodium dithionite (S), sodium cyanide (NaCN), phosphate-buffered saline (PBS), and other chemicals were purchased from Fujifilm Wako.