For Laser Capture Microdissection (LCM) mouse tissues were prepared and frozen in OCT compound and cut to 40-μm sections. Tissue slides were washed with ice-cold RNase-free water for 2 minutes then immediately dehydrated using an ethanol gradient in order to maintain RNA integrity.
For four human specimens, human FFPE sections subsequent to those used for IHC were deparaffinized in ice-cold xylene substitute for 15 minutes and dehydrated in ethanol for 30 seconds. LCM was performed using a PALM MicroBeam system (Zeiss). Microdissected tissues were catapulted into Zeiss AdhesiveCap 200 tubes and stored at −80 °C until RNA extraction.
For four human specimens, human FFPE sections subsequent to those used for IHC were deparaffinized in ice-cold xylene substitute for 15 minutes and dehydrated in ethanol for 30 seconds. LCM was performed using a PALM MicroBeam system (Zeiss). Microdissected tissues were catapulted into Zeiss AdhesiveCap 200 tubes and stored at −80 °C until RNA extraction.