Acetonitrile, chloroform, ethyl acetate and methanol were purchased from J.T.Baker (München, Germany) respectively, d -chloroform, formic acid 98%, Potato Dextrose (PD), Luria-Bertani broth (LB), sodium chloride (NaCl), potassium chloride (KCl), potassium dihydrogen phosphate (KH2PO4), d -methanol, and trifluoroacetic acid (TFA) from Carl Roth GmbH (Karlsruhe, Germany). Bacto malt extract, Bacto peptone and agar were from BD (La Point de Claix, France), d -glucose from Merck (Darmstadt, Germany). Disodium hydrogen phosphate (Na2HPO4) was purchased from J.T.Baker® (The Netherlands), crystal violet from Fluka (Steinheim, Germany), tetracycline and potato dextrose agar (PDA) from Sigma Aldrich (Taufkirchen, Germany) respectively.
D chloroform
Manufactured by Carl Roth
Sourced in Germany
D-chloroform is a clear, colorless liquid used as a solvent in various laboratory applications. It has a characteristic, sweet odor. D-chloroform is a halogenated organic compound with the chemical formula CHCl3.
Automatically generated - may contain errors
Lab products found in correlation
Tetracycline, by Merck Group (3 mentions)
Luria bertani broth, by Carl Roth (3 mentions)
Bacto malt extract, by BD (3 mentions)
D glucose, by Merck Group (3 mentions)
Agar, by BD (3 mentions)
Bacto peptone, by BD (3 mentions)
Trifluoroacetic acid, by Carl Roth (2 mentions)
Disodium hydrogen phosphate na2hpo4, by Avantor (2 mentions)
Potato dextrose, by Carl Roth (2 mentions)
Potato dextrose agar (pda), by Merck Group (2 mentions)
Acetonitrile, by Avantor (1 mentions)
Chloroform, by Avantor (1 mentions)
N hexanoyl l homoserine lactone, by Cayman Chemical (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Formic acid, by Carl Roth (1 mentions)
Wakogel c 200, by Fujifilm (1 mentions)
Dual esi q tof 6520 mass spectrometer, by Agilent Technologies (1 mentions)
Spectrometer, by Bruker (1 mentions)
Silica gel 60 f254 aluminum plates, by Merck Group (1 mentions)
4 protocols using d chloroform
1
Extraction and Analysis of Microbial Metabolites
2
Bacterial Quorum Sensing Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetonitrile, ethyl acetate, chloroform and methanol were purchased from J. T. Baker (USA, Germany and Netherlands respectively), d-chloroform, formic acid 98%, potato dextrose (PD) and Luria-Bertani broth (LB) from Carl Roth GmbH (Karlsruhe, Germany). Bacto malt extract, Bacto peptone and agar were from BD (La Point de Claix, France), D-glucose from Merck (Darmstadt, Germany). N-hexanoyl-L-homoserine lactone was purchased from Cayman Chemical Company (USA), crystal violet from Fluka (Steinheim, Germany), tetracycline and RPMI 1640 medium from Sigma Aldrich (Germany, United Kingdom), respectively.
3
Analytical Reagents for Microbiological Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetonitrile, chloroform, ethyl acetate and methanol were purchased from J. T. Baker (München, Germany) respectively, D-chloroform, formic acid 98%, Casein-soja-peptone (CASO), Potato Dextrose (PD), Luria-Bertani broth (LB), sodium chloride (NaCl), potassium chloride (KCl), potassium dihydrogen phosphate (KH2PO4), D-methanol, and trifluoroacetic acid (TFA) from Carl Roth GmbH (Karlsruhe, Germany). Bacto malt extract, Bacto peptone and agar were from BD (La Point de Claix, France), D-glucose from Merck (Darmstadt, Germany). Disodium hydrogen phosphate (Na2HPO4) was purchased from J.T. Baker® (Deventer, The Netherlands), crystal violet from Fluka (Steinheim, Germany), tetracycline and Potato-Dextrose agar (PDA) from Sigma Aldrich (Taufkirchen, Germany) respectively.
4
Purification and Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemical reagents and solvents were purchased from commercial suppliers (Sigma-Aldrich, St. Louis, MO, USA), TCI-Europe (Zwijdrecht, Belgium) and Merck (Darmstadt, Germany) and were used without purification unless otherwise specified. Deuterated solvents (d-chloroform or d 6 -dimethylsulfoxide) were obtained from Carl Roth GmbH, Karlsruhe, Germany.
The purity of the compounds was monitored by thin layer chromatography (TLC), using silica gel 60 F254 aluminum plates (Merck, Darmstadt, Germany). The visualization was accomplished by UV light. Flash column chromatography was performed using silica gel Wakogel C-200 (Wako Chemical, Osaka, Japan). The melting points of the compounds were determined in open capillaries by using a MEL-TEMP apparatus (Barnstead Thermolyne Corp., Dubuque, IA, USA). NMR spectra were recorded with a Bruker spectrometer ( 1 H-400 MHz and 13 C-100 MHz) in d-chloroform or d 6 -dimethylsulfoxide by using residual solvent signal as an internal standard. HRMS spectra were recorded by a Dual-ESI Q-TOF 6520 mass spectrometer (Agilent Technologies, Wood Dale, IL, USA) at an interface temperature of 350 • C, a desolvation line (DL) temperature of 250 • C, an interface voltage of ±4.500 V and neutral DL/Qarray.
The purity of the compounds was monitored by thin layer chromatography (TLC), using silica gel 60 F254 aluminum plates (Merck, Darmstadt, Germany). The visualization was accomplished by UV light. Flash column chromatography was performed using silica gel Wakogel C-200 (Wako Chemical, Osaka, Japan). The melting points of the compounds were determined in open capillaries by using a MEL-TEMP apparatus (Barnstead Thermolyne Corp., Dubuque, IA, USA). NMR spectra were recorded with a Bruker spectrometer ( 1 H-400 MHz and 13 C-100 MHz) in d-chloroform or d 6 -dimethylsulfoxide by using residual solvent signal as an internal standard. HRMS spectra were recorded by a Dual-ESI Q-TOF 6520 mass spectrometer (Agilent Technologies, Wood Dale, IL, USA) at an interface temperature of 350 • C, a desolvation line (DL) temperature of 250 • C, an interface voltage of ±4.500 V and neutral DL/Qarray.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!