Dibasic sodium hydrogen phosphate

The lONPs (γ-form) were purchased from US Research Nanomaterials Inc (Stock# US7558, Fe₂O₃ CAS# 1309–37-1, Houston, TX). The 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH), H₂O₂, potassium ferricyanide, 1,10-phenanthroline monohydrate, sodium citrate tribasic dihydrate, and ascorbic acid were purchased from Sigma Aldrich (St. Louise, MO). Ammonium bicarbonate, dithiothreitol (DTT), iodoacetamide (IAA), LC/MS-grade water (0.1% v/v formic acid), acetonitrile (0.1% v/v formic acid), monobasic sodium dihydrogen phosphate, sulfuric acid, hydrogen fluoride, dibasic sodium hydrogen phosphate, and the Pierce BCA protein assay kit (Product number: 23227) were purchased from Fisher Scientific (Waltham, MA). Trypsin/Lys-C (mass spectrometry grade) was purchased from Promega (Madison, Wl). Amicon ultra-0.5 centrifugal filter devices (10, 30, 100 kDa cut off membranes) were purchased from Millipore Inc., (Bedford, MA, USA). The 4-aminomethyl-benzylsulfonic acid (ABS) was synthesized according to a published method.^{15 (link)}

The 2,2’-azobis (2-methylpropionamidine) dihydrochloride (AAPH), potassium ferricyanide, 1,10-phenanthroline monohydrate, sodium citrate tribasic dihydrate, and ascorbic acid were purchased from Sigma Aldrich (St. Louis, MO). Ammonium bicarbonate, dithiothreitol (DTT), iodoacetamide (IAA), LC/MS-grade water (0.1% v/v formic acid), acetonitrile (0.1% v/v formic acid), monobasic sodium dihydrogen phosphate, sulfuric acid, hydrogen fluoride, dibasic sodium hydrogen phosphate, and the Pierce BCA protein assay kit (Product number: 23227) were purchased from Fisher Scientific (Waltham, MA). Trypsin from porcine pancreas (Sigma Aldrich, St. Louise, MO) was used for tryptic digestion of the samples. Amicon ultra 0.5 centrifugal filter devices (10, 30, 100 kDa cut off membranes) were purchased from Millipore Inc., (Bedford, MA, USA).