2 3 cgamp tlrl nacga23

Manufactured by InvivoGen

2',3'-cGAMP (tlrl-nacga23) is a synthetic cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) compound. It functions as a cyclic dinucleotide second messenger that activates the stimulator of interferon genes (STING) pathway.

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Lab products found in correlation

Fura 2 am f1221, by Thermo Fisher Scientific (1 mentions) Flag tag f3040, by Merck Group (1 mentions) Ionomycin, by Merck Group (1 mentions) Thapsigargin, by Merck Group (1 mentions) High molecular weight poly 1 c tlrl pic, by InvivoGen (1 mentions) Nac a7250, by Merck Group (1 mentions) Odn 2088, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

2′3′-cGAMP (127 citations) CGAMP (67 citations) 3′3′-cGAMP (16 citations) Tlrl-nacga23 (15 citations) 2′3′-cGAMP (tlrl-nacga23-02) (2 citations)

2 protocols using 2 3 cgamp tlrl nacga23

Protocols for Calcium Signaling Assays

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Fura 2-AM (F1221) was purchased from ThermoFisher Scientific. Thapsigargin and ionomycin were purchased from EMD Millipore. Poly(I:C) (P1530) was purchased from Millipore Sigma. Poly(dA:dT) (tlrl-patn) and 2’,3’-cGAMP (tlrl-nacga23) were purchased from InvivoGen. Tofacitinib (S500110MG) was purchased from Selleck Chemical LLC. Antibodies for detection of STIM1 (5668S), phosphor-IRF3 (29047S), IRF3 (4302S), phosphor-TBK1 (5483S), total TBK1 (3504S), STING (13647S), 6xHis tag (12698S), and STIM2 (4917S) were purchased from Cell Signaling Technologies. Antibodies for detection of FLAG tag (F3040), p58 (ERGIC marker, E1031) and human Orai1 (AB9868) were purchased from Millipore Sigma. Antibody for detection of β-actin (sc-47778) was obtained from Santa Cruz Biotechnology and antibodies for detection of STIM1 (clone 5A2) and GAPDH (GTX100118) from human PBMCs were obtained from Sigma and GeneTex respectively.

Srikanth S., Woo J.S., Wu B., El-Sherbiny Y.M., Leung J., Chupradit K., Rice L., Seo G.J., Calmettes G., Ramakrishna C., Cantin E., An D.S., Sun R., Wu T.T., Jung J.U., Savic S, & Gwack Y. (2019). The Ca2+ sensor STIM1 regulates type I interferon response by retaining the signaling adaptor STING at the endoplasmic reticulum. Nature immunology, 20(2), 152-162.

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Stimulation of Innate Immune Responses

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LPS from Escherichia coli, serotype EH100 (ALX-581–010-L001), was purchased from Enzo Life Sciences. High molecular weight poly(I:C) (tlrl-pic) and 2′−3′-cGAMP (tlrl-nacga23) were purchased from Invivogen. Recombinant mouse IFNβ1 (581302) and recombinant mouse IL-10 (417-ML-005/CF) were purchased from BioLegend. ATP disodium salt (A2383), DMSO (D8418), AOAA (C13408), valinomycin (V3639), TAM (H6278) and NAC (A7250) were purchased from Sigma Aldrich. Oligomycin A from Streptomyces diastatochromogenes (M02220) was purchased from Fluorochem. FHIN1 (HY-100004), DMF (HY-17363), MMF (HY-103252), IMT1 (HY-134539) and C-178 (HY-123963) were purchased from MedChemExpress. CPG ODN 1826 (130–100-274) and ODN 2088 (130–105-815) were purchased from Miltenyi Biotec. CCCP (M20036) was purchased from Thermo Fisher.

Hooftman A., Peace C.G., Ryan D.G., Day E.A., Yang M., McGettrick A.F., Yin M., Montano E.N., Huo L., Toller-Kawahisa J.E., Zecchini V., Ryan T.A., Bolado-Carrancio A., Casey A.M., Prag H.A., Costa A.S., De Los Santos G., Ishimori M., Wallace D.J., Venuturupalli S., Nikitopoulou E., Frizzell N., Johansson C., Von Kriegsheim A., Murphy M.P., Jefferies C., Frezza C, & O’Neill L.A. (2023). Macrophage fumarate hydratase restrains mtRNA-mediated interferon production. Nature, 615(7952), 490-498.

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