Glycated albumin (MGA) was prepared as described (50 (link)). Briefly, 500 μM methylglyoxal (Sigma, St. Louis, MO, US) was incubated with 100 uM BSA (Sigma) dissolved in phosphate buffered saline (PBS) for 24 hours, then washed on 10 kDa filters (Macrosep® Advance Device, Pall Life Sciences, MI, USA) to remove excess methyl glyoxal, reconstituted with DMEM/F12 serum free media and passed through a 0.2 μmicron filter. TGF-β (5 nanograms (ng)/ml, Peprotech, NJ, USA) was prepared according to manufacturer to treat cells in a subset of experiments. Cells were co-treated with one or combinations of the following: Ang-(1-7) (100 nM, Bachem, CA, USA), D-Ala7-Ang-(1-7) (10 μM, DAL, Bachem), ERK1/2 kinase inhibitor, PD 98059 (1 μM, PD, Sigma), TGF- β receptor kinase inhibitor; SB525334 (1 μM, SB, Selleckchem, TX, USA), the AT1 receptor antagonist losartan (1 μM, Merck Pharmaceuticals, NJ, USA), the renin inhibitor aliskerin (1 μM, Tocris) and the ACE inhibitor lisinopril (1 μM, Sigma).
D ala7 ang 1 7
Manufactured by Bachem
Sourced in United States
D-Ala7-Ang-(1-7) is a synthetic peptide product manufactured by Bachem. It is a heptapeptide with the sequence H-Ala-Arg-Val-Tyr-Ile-His-Pro-OH. The core function of this product is for use in research and scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Ang 1 7, by Bachem (2 mentions)
Lisinopril, by Merck Group (1 mentions)
Sb525334, by Selleck Chemicals (1 mentions)
Pd98059, by Merck Group (1 mentions)
Tgf β, by Thermo Fisher Scientific (1 mentions)
Macrosep advance device, by Pall Corporation (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Methylglyoxal, by Merck Group (1 mentions)
Losartan, by Merck Group (1 mentions)
Methylated bovine serum albumin (mbsa), by Merck Group (1 mentions)
Complete freund adjuvant (cfa), by Merck Group (1 mentions)
2 protocols using d ala7 ang 1 7
1
Glycated Albumin and Angiotensin Receptor Modulation
2
Induction and Modulation of Experimental Arthritis
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To induce arthritis, animals were immunized with an intradermal injection of 100 µg of methylated bovine albumin (mBSA, Sigma, St. Louis, MO, USA), emulsified in 500 µg of Freund’s complete adjuvant (CFA, Sigma) at the base of the tail (day 0). Two weeks after immunization, antigen challenge was performed by injection of 10 µg of mBSA diluted in 10 µl of sterile saline into the left joint. As control group received intra-articular injection of PBS (10 µl) into the same site. Mice were treated with an intra-articular injection (10 µl) of 100 ng of Ang-(1-7) (Bachem, Torrance, CA, USA), Mas antagonist, A779 [D-Ala7-Ang-(1-7), 200 ng/cavity; Bachem, Torrance, CA, USA], or the vehicle (NaCl 0.9%) 12 h after antigen challenge (26 (link)). All surgical procedures were performed under ketamine and xylazyne anesthesia (150 and 10 mg/kg, respectively) followed by euthanasia.
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