Anti cd59 fitc

Ten milliliters of peripheral blood was collected from PNH patients into an ethylenediaminetetraacetic acid (EDTA) anticoagulant tube. First, the supernatants were discarded after centrifugation (3 g, 5 min), and the remaining blood was separated into five centrifuge tubes. After 15 min, the erythrocyte lysate was centrifuged again and washed with PBS. Anti-CD59 FITC (BD PharMingen, USA) was added and incubated for 20 min in a dark room. Furthermore, to obtain CD59⁻ and CD59⁺ granulocytes and monocytes, PNH clones were sorted by flow cytometry (BD FACSAria II, USA). The separation purity was greater than 90% (Fig. 1a, b, c).Fig. 1

a The cells of PNH patients were sorted by flow cytometry to obtain CD59⁻ and CD59⁺ granulocytes and monocytes. b Purity of CD59⁻ granulocytes and monocytes after sorting. c Purity of the CD59⁺ granulocytes and monocytes after sorting. The sorting purity was greater than 90%