Before making plates, appropriate amount of individual hormone solutions were added into the culture medium around 50 °C. Jasmonic acid (Sigma), (S)-(+)-Ibuprofen (Sigma), Paclobutrazol (Sigma), Gibberellic acid (Sigma), Ancymidol (Sigma) were dissolved in ethanol. Aminoethoxyvinyl glycine hydrochloride (Sigma), AgNO3 (Sinopharm Chemical Reagent Co., LTD), 2,3,5-triiodobenzoic acid (Sigma) and Diethyldithiocarbamic acid (Sigma) were dissolved in ddH2O.
S ibuprofen
Manufactured by Merck Group
Sourced in United States
S-ibuprofen is a chiral isomer of the non-steroidal anti-inflammatory drug (NSAID) ibuprofen. It is a white crystalline powder that serves as a key intermediate in the synthesis of pharmaceutical products.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (2 mentions)
Gibberellic acid, by Merck Group (1 mentions)
Jasmonic acid, by Merck Group (1 mentions)
Diethyldithiocarbamic acid, by Merck Group (1 mentions)
Paclobutrazol, by Merck Group (1 mentions)
2 3 5 triiodobenzoic acid, by Merck Group (1 mentions)
Agno3, by Sinopharm (1 mentions)
D glucose, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Merck Group (1 mentions)
Cocl2 6h2o, by Merck Group (1 mentions)
Naoh pellets, by Thermo Fisher Scientific (1 mentions)
Mgcl2 6h2o, by Merck Group (1 mentions)
Sodium hydroxide solution, by Thermo Fisher Scientific (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Ammonium formate, by Merck Group (1 mentions)
R s propranolol, by Merck Group (1 mentions)
R s salbutamol, by Merck Group (1 mentions)
R s fluoxetine, by Merck Group (1 mentions)
5 protocols using s ibuprofen
1
Hormone Application in Tissue Culture
2
S-Ibuprofen Extraction and Quantification
3
Chiral Separation and Quantification Protocol
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Methanol, ammonium acetate, acetic acid and ammonium formate were HPLC grade and obtained from Sigma Aldrich. The reference materials R/S(±)-naproxen, R(-)-naproxen, S(+)-naproxen, R/S(±)ibuprofen, R(-)-ibuprofen, S(+)-ibuprofen, R/S(±)-salbutamol, R/S(±)-bisoprolol, R/S(±)-metoprolol, R/S(±)-amphetamine, S(+)-amphetamine, R(-)-amphetamine, R/S(±)-propranolol, R/S(±)-acebutolol, R/S(±)-fluoxetine, R/S(±)-atenolol, R(+)-atenolol, S(-)-atenolol R/S(±)-chlorpheniramine, and R/S(±)citalopram were purchased from Sigma Aldrich (Gillingham, UK) and Toronto Research Chemicals (Toronto, Canada). The corresponding deuterated surrogates were also purchased as racemates: R/S(±)-
atenolol-d7, R/S(±)-chlorpheniramine-d6, and R/S(±)-citalopram-d6. All chemicals were purchased as Methanolic solutions of 0.1 mg mL -1 or 1 mg mL -1 , or as powder. Powders were prepared at an appropriate concentration in Methanol. All solutions were stored in the dark at -20°C. Oasis HLB cartridges (3cc 60mg) were purchased from Waters (Manchester, UK).
atenolol-d7, R/S(±)-chlorpheniramine-d6, and R/S(±)-citalopram-d6. All chemicals were purchased as Methanolic solutions of 0.1 mg mL -1 or 1 mg mL -1 , or as powder. Powders were prepared at an appropriate concentration in Methanol. All solutions were stored in the dark at -20°C. Oasis HLB cartridges (3cc 60mg) were purchased from Waters (Manchester, UK).
4
Preparation and Application of Drug Compounds
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Drugs used in this study included azithromycin (Sigma‐Aldrich), IL‐1Ra (PeproTech), Infliximab (Sigma‐Aldrich), LPS‐RS (InvivoGen), TAK‐242 (Sigma‐Aldrich), MCC950 (Sigma‐Aldrich) and S+‐ibuprofen (Sigma‐Aldrich; Supplementary table 3 ). Compounds were dissolved in sterile dimethyl sulfoxide, 0.1% solution of bovine serum albumin or ultra‐pure water according to manufacturer’s recommendations (Supplementary table 3 ). For tests of compounds in neutrophil transmigration assays, a small volume of drug was added to aliquots of ALI co‐infection washes according to concentrations used in prior in vitro studies (Supplementary table 3 ).
5
Solubility Enhancement of NSAIDs
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Ketoprofen, ketorolac, indoprofen, fenoprofen, suprofen, ibuprofen, S-ibuprofen, flurbiprofen, benzoic acid, naphthalene, and uracil standard were purchased from Sigma-Aldrich (St. Louis, MO, USA). Naproxen reference standard was kindly provided by Temad faculty (Tehran, Iran).
The chemical structures of solutes have been summarized in Fig. 1S. Some chemical and physical properties of tested solutes are collected in table 1. Hp-β-CD with the substitution degree of 3.5-5.5 was obtained from Cyclodextrin-Shop (Tilburg, Netherlands). Purified water from a Milli-Q reagent water system (Millipore, Bedford, MA, USA) was used to prepare the buffer and reagent solutions. The analytical grade H3PO4, NaH2PO4, NaOH were purchased from Merck (Darmstadt, Germany). LC/MS grade acetonitrile, ethanol and methanol were also purchased from Biosolve (Valkenswaard, Netherland).
The chemical structures of solutes have been summarized in Fig. 1S. Some chemical and physical properties of tested solutes are collected in table 1. Hp-β-CD with the substitution degree of 3.5-5.5 was obtained from Cyclodextrin-Shop (Tilburg, Netherlands). Purified water from a Milli-Q reagent water system (Millipore, Bedford, MA, USA) was used to prepare the buffer and reagent solutions. The analytical grade H3PO4, NaH2PO4, NaOH were purchased from Merck (Darmstadt, Germany). LC/MS grade acetonitrile, ethanol and methanol were also purchased from Biosolve (Valkenswaard, Netherland).
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