Fluorescein isothiocyanate–dextran (FITC-dextran, 66,100 Da) was injected (40 mg/kg) intravenously into BALB/c mice. U46619 (20 μg diluted in methyl acetate) was applied onto the auricle. The vasculature of mice ear was observed every 5 min with confocal microscope (ECLIPSE Ti with C1 confocal system, Nikon, Tokyo, Japan). FITC-dextran leakage and vascular diameter of arteries and veins were quantified as described previously with EZ-C1 FreeViewer (Nikon, Tokyo, Japan)32 (link).
Eclipse ti with c1 confocal system
Manufactured by Nikon
Sourced in Japan
The ECLIPSE Ti with C1 confocal system is a laboratory equipment designed for high-resolution optical imaging. It features a confocal scanning system that enables the acquisition of sharp, high-contrast images by reducing out-of-focus light. The system is compatible with a range of sample types and can be used for various applications in life science research and other scientific disciplines.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using eclipse ti with c1 confocal system
1
Quantifying Vascular Permeability and Diameter
2
Visualizing Mouse Ear Vasculature
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To visualize mouse ear vessels using a confocal microscope (ECLIPSE Ti with C1 confocal system, Nikon, Tokyo, Japan), 70 kDa fluorescein isothiocyanate-dextran (10 mg/kg in 100 μl phosphate-buffered saline, Sigma-Aldrich, St. Louis, MO, USA) was injected intravenously. Mice were then positioned on the microscope stage and their body temperatures were maintained at 37°C. Dextran leakage and vascular diameter were monitored every minute and quantified as described previously [13 (link)] using EZ-C1 FreeViewer (Nikon, Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!