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Ab207203

Manufactured by Abcam
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Ab207203 is a monoclonal antibody that recognizes the Myc-tag epitope. The antibody is designed for use in various laboratory techniques, such as Western blotting, immunoprecipitation, and immunocytochemistry, to detect and study proteins fused with the Myc-tag.

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Lab products found in correlation

Ab14860, by Abcam (2 mentions) S rbd, by ACROBiosystems (2 mentions) S trimer, by ACROBiosystems (2 mentions)

2 protocols using ab207203

1

Evaluating ERα Activity in MCF-7 Cells

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The ERα activity was determined by the ERα transcription factor activation assay kit (Abcam, ab207203) according to the manufacturer’s directions. Briefly, MCF-7 nuclear extracts (5 μg; Abcam, ab14860) were treated with S (0.01 to 300 nM; ACROBiosystems), S-RBD (1 to 100 nM; ACROBiosystems), S-trimer (1 to 100 nM; provided by M. Borgnia), and/or E2 (100 nM: Tocris). Extracts were added to each well coated with the ER consensus binding site (5′-GGTCACAGTGACC-3′). The wells were washed and then incubated with rabbit anti-ERα (1:2000; 1 hour at RT) and horseradish peroxidase (HRP)–conjugated secondary antibody (1:2000; 1 hour at RT) that were provided with the kit. Colorimetric reaction was measured by spectrophotometry at a wavelength of 450 nm. Values were given as percentage of the effect induced by E2.
Solis O., Beccari A.R., Iaconis D., Talarico C., Ruiz-Bedoya C.A., Nwachukwu J.C., Cimini A., Castelli V., Bertini R., Montopoli M., Cocetta V., Borocci S., Prandi I.G., Flavahan K., Bahr M., Napiorkowski A., Chillemi G., Ooka M., Yang X., Zhang S., Xia M., Zheng W., Bonaventura J., Pomper M.G., Hooper J.E., Morales M., Rosenberg A.Z., Nettles K.W., Jain S.K., Allegretti M, & Michaelides M. (2022). The SARS-CoV-2 spike protein binds and modulates estrogen receptors. Science Advances, 8(48), eadd4150.
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2

Assay of ERα Transcriptional Activity

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The ERα activity was determined by the ERα transcription factor activation assay kit (ab207203, Abcam) according to manufacturer’s directions. Briefly, MCF-7 nuclear extracts (5 μg; ab14860, Abcam) were treated with either S (0.01–300 nM; Acro Biosystems) S-RBD (1–100 nM; Acro Biosystems), S-trimer (1–100 nM; provided by Dr. Borgnia) and/or E2 (100 nM: Tocris). Extracts were added to each well coated with the ER consensus binding site (5’ – GGTCACAGTGACC – 3’). The wells were washed and then incubated with rabbit anti-ERα (1:2000, 1 h, RT) and horseradish-conjugated secondary antibody (1:2000, 1 h, RT) that were provided with the kit. Colorimetric reaction was measured by spectrophotometry at a wavelength of 450 nm.
Solis O., Beccari A.R., Iaconis D., Talarico C., Ruiz-Bedoya C.A., Nwachukwu J.C., Cimini A., Castelli V., Bertini R., Montopoli M., Cocetta V., Borocci S., Prandi I.G., Flavahan K., Bahr M., Napiorkowski A., Chillemi G., Ooka M., Yang X., Zhang S., Xia M., Zheng W., Bonaventura J., Pomper M.G., Hooper J.E., Morales M., Rosenberg A.Z., Nettles K.W., Jain S.K., Allegretti M, & Michaelides M. (2022). The SARS-CoV-2 spike protein binds and modulates estrogen receptors. bioRxiv.
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