Rotavapor 4001 efficient

MAE was performed using a method previously reported by Batinić et al. [17 (link)]. MAE was carried out at 100 °C using a microwave reactor (Milestone ETHOS X, 2.45 GHz, Milestone, Italy) equipped with a digital infrared temperature sensor that monitors the process temperature and two magnetrons with a maximum operative power of 1.8 kW. All experiments were conducted in three cycles, in the SR-15 rotor with a power of 0.7 kW, in a closed poly(tetrafluoroethylene) Teflon vessel (0.1 L), using a magnetic stirring bar (12 × 30 mm) for uniform mixing (the mixing speed was software-controlled; magnetic stirring of solution up to a speed of 3400 rpm). Ethyl alcohol with 50% water, an extraction time of 2 min, and a solid-to-solvent ratio of 1:10 were employed in the MAE. Following the extraction process, permeate was separated from retentate using a laboratory glass funnel and cellulose-acetate filter (25 mm, 0.45 µm). The solvent was removed using vacuum evaporation (Rotavapor Heidolph 4001-efficient, Heidolph Instruments, Schwabach, Germany) at a temperature of 45 °C and a pressure of 0.180 ± 0.05 bar. The extracts were stored in the refrigerator at 4 °C before analysis.