Iraffinity 1s fourier transform infrared ftir spectrometer

To determine the successful encapsulation of luteolin molecules within ZIF-8 nanoparticles, the surface morphologies of ZIF-8 and luteolin@ ZIF-8 nanoparticles were examined utilizing a H-7800 transmission electron microscope (TEM, Hitachi, Japan). Their composition was analyzed by an IRAffinity-1S fourier transform infrared (FTIR) spectrometer (Shimadzu, Japan). Particle sizes and zeta potentials were determined employing a Zetasizer Nano ZS laser particle size analyzer (Malvern, UK). The phase structure of ZIF-8 and luteolin@ ZIF-8 nanoparticles was elucidated through D8 Advance X-ray diffraction (XRD, Thermo Fisher, USA).

The removal of the template from the MIP was determined
through thermal gravimetric analysis (TGA) using a TA Instruments
TGA 550 Auto Advanced. Measurements were performed under a nitrogen
atmosphere at a heating rate of 10 °C/min. For each measurement,
2.5–4 mg of the polymer sample was used. The amount of polymers
used for each measurement was between 2.5 and 4 mg. Further confirmation
of template removal was conducted with an IR-Affinity-1S Fourier transform
infrared (FTIR) spectrometer (Shimadzu Corp., Kyoto, Japan) coupled
to an attenuated total reflectance (ATR) crystal, comparing the spectra
of extracted, non-extracted, and GA samples. The instrument was set
up to run 32 scans per measurement with a spectral resolution of 4
cm^–1. The IR spectra were recorded between 4000
and 400 cm^–1. The ATR crystal was cleaned with ethanol
70% v/v and acetone before starting the measurement for each new sample.
A background spectrum was taken before measuring every new sample
to account for environmental changes.